Structure and bioactivity of polysaccharide from a subseafloor strain of Schizophyllum commune 20R-7-F01
Structure and bioactivity of polysaccharide from a subseafloor strain of Schizophyllum commune 20R-7-F01
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裂褶菌 20R-7-F01 海底菌株多糖的结构和生物活性
DOI：
10.1016/j.ijbiomac.2022.09.189
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发表时间：
2022
影响因子：
8.2
通讯作者：
Changhong Liu
中科院分区：
文献类型：
--
作者：
Yunan Ma;Zhen Wang;Muhammad Zain Ul Arifeen;Yarong Xue;Sheng Yuan;Changhong Liu
真菌多糖是一种具有多种生物活性的生物大分子，具有广泛的应用前景，可能在生物应对极端环境中发挥重要作用。在此，我们报道了一种胞外多糖（EPS），该多糖是由裂叶菌公社20R-7-F01产生的，从海底2公里以下的海底沉积物中分离出来，这些沉积物是在远征337期间获得的。EPS的单糖为葡萄糖，分子量为608.8 kDa。甲基化和核磁共振分析表明，EPS的主链为（1→3）-β- d -葡聚糖，每3个残基上有一个侧链（1→6）-β- d -葡聚糖连接。生物活性实验表明，EPS具有较强的抗氧化活性，能促进RAW264.7细胞的活性和吞噬能力。这些结果表明，来自海底沉积物的真菌是重要的多糖新来源，可能参与了真菌对缺氧海底极端生态系统的适应。
Fungal polysaccharide is a kind of biomacromolecule with multiple biological activities, which has a wide application prospect and may play an important role in organisms to cope with extreme environments. Herein, we reported an extracellular polysaccharide (EPS) produced by Schizophyllum commune 20R-7-F01 that was isolated from subseafloor sediments at ~2 km below the seafloor, obtained during expedition 337. The monosaccharide of EPS was glucose and its molecular weight was 608.8 kDa. Methylation and NMR analysis indicated that the backbone of the EPS was (1 → 3)-β-D-glucan with a side chain (1 → 6) β-D-glucan linking at every third residue. Bio-active assays revealed that the EPS had potent antioxidant activity and could promote RAW264.7 cells viability and phagocytosis. These results suggest that fungi derived from sediments below seafloor are important and new source of polysaccharides and may be involved in the adaptation of fungi to anoxic subseafloor extreme ecosystem.