Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (dbs) samples
Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (dbs) samples
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DOI：
10.1590/s0036-46652008000300004
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发表时间：
2008-06-01
期刊：
影响因子：
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通讯作者：
D'Azevedo, Pedro Alves
中科院分区：
文献类型：
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作者：
Castro, Andréa Cauduro de;Borges, Luiz Gustavo dos Anjos;D'Azevedo, Pedro Alves
对457份干全血斑点标本（S&S 903）进行了人类免疫缺陷病毒1型和2型抗体检测。使用Q-Preven HIV 1+2作为筛选试验。并与ELISA金标准血清检测结果进行比较（Cobas Core e Axsym HIV 1/2 gO）和免疫荧光是确定性确认试验。样品从巴西RS的波尔图阿莱格雷的Nossa Senhora da Conceicao医院获得，通过全血转移至滤纸卡并送至南卡希亚斯，RS -巴西，在那里进行测试。用两个不同的板评价干全血斑点的稳定性。第一个由储存在室温、4 ℃、-20 ℃和-70 ℃下的5个阴性和5个阳性样本组成，而第二个由储存在37 ℃（湿度）下的2个阴性和3个阳性样本组成
Human Immunodeficiency Virus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV 1/2 gO) and imunofluorescence was the definitive confirmatory test.The samples were obtained from the Hospital Nossa Senhora da Conceicao in Porto Alegre, RS - Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul, RS - Brazil where the tests were performed.The dried whole blood spot stability was evaluated with two different panels. The first one was composed of five negative and five positive samples stored at room temperature, 4 degrees C, -20 degrees C and -70 degrees C, while the second was composed of two negative and three positive samples stored at 37 degrees C (humidity