Histamine induces chemotaxis and phagocytosis in murine bone marrow-derived macrophages and RAW 264.7 macrophage-like cells via histamine H4-receptor
Histamine induces chemotaxis and phagocytosis in murine bone marrow-derived macrophages and RAW 264.7 macrophage-like cells via histamine H4-receptor
复制标题
DOI：
10.1007/s00011-013-0694-0
复制
发表时间：
2014-03-01
影响因子：
6.7
通讯作者：
Neumann, Detlef
中科院分区：
文献类型：
--
作者：
Czerner, Christoph P.;Klos, Andreas;Neumann, Detlef
为探讨组胺H-4受体在小鼠巨噬细胞上的表达及其功能，采用逆转录聚合酶链反应（RT-PCR）技术，从mRNA水平研究了组胺H-4受体在BALB/c小鼠骨髓巨噬细胞和RAW264.7细胞上的表达模式。通过分析组胺诱导的趋化性和吞噬作用，研究了组胺受体的功能相关性（组胺H-1受体），法莫替丁（组胺H-2受体），thioperamide（组胺H-3/4受体）和JNJ 7777120骨髓来源的巨噬细胞和RAW 264.7细胞都表达组胺H-1受体和组胺H-4受体的mRNA。仅在骨髓源性巨噬细胞中发现组胺H-2受体mRNA的残留量。在两种细胞模型中，组胺诱导趋化性和吞噬活性，硫代哌丁胺和JNJ 7777120可降低该活性，但美托咪胺或法莫替丁不会降低该活性。在小鼠骨髓源性巨噬细胞和RAW 264.7巨噬细胞样细胞中，组胺H-4受体介导趋化性和吞噬活性。
Expression and function of histamine H-4-receptor, an immunomodulatory receptor involved in inflammatory diseases, on murine macrophages, which are vital for immunity, were investigated.The expression pattern of histamine receptors on bone marrow-derived macrophages of BALB/c mice and on RAW 264.7 cells was studied at the mRNA level by reverse transcription polymerase chain reaction. The functional relevance of histamine receptors was investigated by analyzing histamine-induced chemotaxis and phagocytosis in the presence of histamine receptor antagonists mepyramine (histamine H-1-receptor), famotidine (histamine H-2-receptor), thioperamide (histamine H-3/4-receptors) and JNJ7777120 (histamine H-4-receptor).Both bone marrow-derived macrophages and RAW 264.7 cells express mRNA for histamine H-1-receptor and histamine H-4-receptor. Residual amounts of histamine H-2-receptor mRNA are found in bone marrow-derived macrophages only. In both cellular models, histamine induced chemotaxis and phagocytic activity, which was reduced by thioperamide as well as by JNJ 7777120, but not by mepyramine or famotidine.In murine bone marrow-derived macrophages and RAW 264.7 macrophage-like cells histamine H-4-receptor mediates chemotaxis and phagocytic activity.