Immunohistochemical studies on the development of tyrosine hydroxylase- and serotonin-immunoreactive neurons in fetal dorsal raphe tissue transplanted into the anterior eye chamber of adult rats.
Immunohistochemical studies on the development of tyrosine hydroxylase- and serotonin-immunoreactive neurons in fetal dorsal raphe tissue transplanted into the anterior eye chamber of adult rats.
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对移植到成年大鼠前眼房的胎儿中缝背侧组织中酪氨酸羟化酶和血清素免疫反应性神经元发育的免疫组织化学研究。
DOI：
10.1679/aohc.52.553
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发表时间：
1989
影响因子：
--
通讯作者：
Y. Sano
中科院分区：
文献类型：
--
作者：
T. Tanabe;S. Ueda;Y. Sano
将含有胎鼠脑中缝背核的组织块移植到成年大鼠的眼前房中。用酪氨酸羟化酶（TH）和5-羟色胺抗血清对移植物中的儿茶酚胺能神经元和肾上腺素能神经元的发育模式（尤其是轴突突起的延伸）之间的差异进行了免疫化学检查。在移植后的早期阶段（3天），TH-阳性神经元出现在移植物，并没有与单胺氧化酶抑制剂（MAOI，Nialamide）预处理，而阿托宁阳性神经元仅表现在移植物经历了MAOI预处理。TH和5-羟色胺神经元之间的实验环境中的生长模式的形态差异也被证明：在这个早期阶段，TH神经元的胞体是多极和星状的，并具有几个不同的过程，而5-羟色胺神经元是卵形的，缺乏这样的过程。移植后1周至1个月，TH阳性纤维逐渐增多，但仅分布于移植物内TH神经元胞体周围。移植物内5-羟色胺神经元突起形成密集的神经丛，移植后1周有少量5-羟色胺神经元突起伸入宿主虹膜。手术后一个月，5-羟色胺纤维的密度在整个移植物中逐渐增加，突出的5-羟色胺纤维在宿主葡萄膜组织中形成了静脉曲张纤维网络。（250字处删节）
Pieces of tissue containing dorsal raphe nuclei of fetal rat brains were transplanted into the anterior eye chambers of adult rats. The differences between the developmental patterns of catecholaminergic and serotonergic neurons--especially the extension of their axonal processes--in the grafts were immunohistochemically examined using tyrosine hydroxylase (TH) and serotonin antisera. At an early stage after transplantation (3 days), TH-positive neurons appeared in grafts that had and had not been pretreated with a monoamine oxidase inhibitor (MAOI, Nialamide), while serotonin-positive neurons were demonstrated only in the grafts that had undergone MAOI pretreatment. Morphological differences in the growth pattern in the experimental milieu between the TH and serotonin neurons were also demonstrated: at this early stage, the somata of the TH neurons were multipolar and stellate shaped and possessed several distinct processes, while the serotonin neurons were ovoid shaped and lacked such processes. One week to 1 month after transplantation, the number of TH-positive fibers gradually increased, but their distribution was restricted to the area surrounding the cell bodies of the TH neurons in the graft. However, the processes of the serotonin neurons formed a dense plexus in the graft, and a small number of these fibers extended into the host iris 1 week after transplantation. By one month after the operation, the density of the serotonin fibers had gradually increased throughout the graft, and protruding serotonin fibers formed a network of varicose fibers in the host uveal tissue.(ABSTRACT TRUNCATED AT 250 WORDS)