Differential photoinhibition of bacterial and archaeal ammonia oxidation
Differential photoinhibition of bacterial and archaeal ammonia oxidation
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DOI：
10.1111/j.1574-6968.2011.02457.x
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发表时间：
2012-02-01
影响因子：
2.1
通讯作者：
Prosser, James I.
中科院分区：
文献类型：
--
作者：
Merbt, Stephanie N.;Stahl, David A.;Prosser, James I.
光的抑制可能会影响氨氧化剂在水生环境中的分布，是一种解释亚硝酸盐的最大值附近的海洋沃茨的透光区的基础。以前的光抑制研究一直局限于细菌氨氧化剂，而不是古细菌氨氧化剂，占主导地位的海洋环境。为了比较细菌和古细菌氨氧化剂的光抑制作用，在连续光照和光/暗循环条件下，测定了两种氨氧化古细菌（Nitrosopumilus maritimus和Nitrosotalea devanaterra）和细菌（Nitrosomonaseuropaea和Nitrosospira multiformis）在不同光强下的比生长速率。所有菌株都受到最高强度连续光照的抑制（500类似于mu E类似于m-2类似于s-1）。在较低的光强度下，古细菌的生长比细菌的生长更敏感，在60类似于mu E类似于m-2类似于s-1的抑制比在15类似于mu E类似于m-2类似于s-1的抑制更大，其中细菌不受影响。微生物氨氧化剂也更敏感的周期，8小时的光/16小时的黑暗中，在两个光强度（60和15类似于亩E类似于M-2类似于S-1），不像细菌菌株，没有证据表明在黑暗阶段的恢复。研究结果提供了证据，生态位分化在水生环境中，减少光抑制的解释在海洋中的亚硝酸盐最大值的支持。
Inhibition by light potentially influences the distribution of ammonia oxidizers in aquatic environments and is one explanation for nitrite maxima near the base of the euphotic zone of oceanic waters. Previous studies of photoinhibition have been restricted to bacterial ammonia oxidizers, rather than archaeal ammonia oxidizers, which dominate in marine environments. To compare the photoinhibition of bacterial and archaeal ammonia oxidizers, specific growth rates of two ammonia-oxidizing archaea (Nitrosopumilus maritimus and Nitrosotalea devanaterra) and bacteria (Nitrosomonas europaea and Nitrosospira multiformis) were determined at different light intensities under continuous illumination and light/dark cycles. All strains were inhibited by continuous illumination at the highest intensity (500 similar to mu E similar to m-2 similar to s-1). At lower light intensities, archaeal growth was much more photosensitive than bacterial growth, with greater inhibition at 60 similar to mu E similar to m-2 similar to s-1 than at 15 similar to mu E similar to m-2 similar to s-1, where bacteria were unaffected. Archaeal ammonia oxidizers were also more sensitive to cycles of 8-h light/16-h darkness at two light intensities (60 and 15 similar to mu E similar to m-2 similar to s-1) and, unlike bacterial strains, showed no evidence of recovery during dark phases. The findings provide evidence for niche differentiation in aquatic environments and reduce support for photoinhibition as an explanation of nitrite maxima in the ocean.