Method using in vivo quantitative spectroscopy to guide design and optimization of low-cost, compact clinical imaging devices: emulation and evaluation of multispectral imaging systems.
Method using in vivo quantitative spectroscopy to guide design and optimization of low-cost, compact clinical imaging devices: emulation and evaluation of multispectral imaging systems.
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DOI：
10.1117/1.jbo.23.4.046002
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发表时间：
2018-04
影响因子：
3.5
通讯作者：
Durkin AJ
中科院分区：
文献类型：
--
作者：
Saager RB;Baldado ML;Rowland RA;Kelly KM;Durkin AJ
随着最近紧凑和/或低成本临床多光谱成像方法和商业可用组件的激增，问题仍然存在，它们是否充分捕获其应用所需的光谱内容。我们提出了一种方法来模拟各种多光谱成像仪的光谱范围和分辨率，在体内的数据从空间频域光谱（SFDS）的基础上。该方法模拟了400至1100 nm的光谱响应。将仿真数据与体内组织的完整SFDS光谱进行比较提供了评估这些成像器的稀疏光谱内容是否能够（1）解释存在的所有光学对比度源（完整性）以及（2）稳健地分离和量化光学对比度源（串扰）的机会。我们验证了一系列的组织模拟幻影的方法，比较SFDS为基础的仿真光谱对测量从一个独立的特征多光谱成像仪。仿真结果在所有体模中与成像仪匹配（吸收，减少散射）。体内测试案例（烧伤和光老化）说明了SFDS如何用于评估不同的多光谱成像仪。这种方法提供了一种体内测量方法，以评估特定于其目标临床应用的多光谱成像仪的性能，并可以帮助设计和优化新的光谱成像设备。
With recent proliferation in compact and/or low-cost clinical multispectral imaging approaches and commercially available components, questions remain whether they adequately capture the requisite spectral content of their applications. We present a method to emulate the spectral range and resolution of a variety of multispectral imagers, based on in-vivo data acquired from spatial frequency domain spectroscopy (SFDS). This approach simulates spectral responses over 400 to 1100 nm. Comparing emulated data with full SFDS spectra of in-vivo tissue affords the opportunity to evaluate whether the sparse spectral content of these imagers can (1) account for all sources of optical contrast present (completeness) and (2) robustly separate and quantify sources of optical contrast (crosstalk). We validate the approach over a range of tissue-simulating phantoms, comparing the SFDS-based emulated spectra against measurements from an independently characterized multispectral imager. Emulated results match the imager across all phantoms ( absorption, reduced scattering). In-vivo test cases (burn wounds and photoaging) illustrate how SFDS can be used to evaluate different multispectral imagers. This approach provides an in-vivo measurement method to evaluate the performance of multispectral imagers specific to their targeted clinical applications and can assist in the design and optimization of new spectral imaging devices.