Does Cry1Ab protein affect learning performances of the honey bee Apis mellifera L. (Hymenoptera, Apidae)?
Does Cry1Ab protein affect learning performances of the honey bee Apis mellifera L. (Hymenoptera, Apidae)?
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DOI：
10.1016/j.ecoenv.2007.12.002
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发表时间：
2008-06
影响因子：
6.8
通讯作者：
R. Ramírez-Romero;Nicolas Desneux;A. Decourtye;Antoine Chaffiol;M. Pham-Delègue
中科院分区：
文献类型：
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作者：
R. Ramírez-Romero;Nicolas Desneux;A. Decourtye;Antoine Chaffiol;M. Pham-Delègue
转基因Bt作物在世界范围内越来越多地使用，但副作用，特别是对益虫的亚致死作用仍然缺乏研究。蜜蜂是通过授粉对自然和人工生态系统有益的昆虫。本研究的目的是评估两种浓度的Cry1Ab蛋白（3和5000 ppb）对年轻成年蜜蜂的潜在影响。在补充生物测定之后，我们的实验评估了Cry1Ab对年轻成年蜜蜂的三个主要生命特征的影响：（a）在亚慢性暴露于Cry1Ab期间蜜蜂的存活，（B）进食行为，和（c）蜜蜂成为觅食者时的学习表现。后者的效果进行了测试，使用长鼻伸展反射（PER）的程序。同样的效果，也测试了使用化学农药，吡虫啉，作为阳性参考。试验浓度的Cry1Ab蛋白对蜜蜂没有致死作用。然而，当暴露于最高浓度的Cry1Ab蛋白时，蜜蜂的摄食行为受到影响，蜜蜂需要更长的时间来吸收受污染的糖浆。此外，暴露于5000ppb的Cry1Ab的蜜蜂具有干扰的学习表现。即使没有食物奖励，蜜蜂也会继续对条件气味做出反应。我们的研究结果表明，转基因作物表达Cry1Ab蛋白在5000 ppb可能会影响食物消费或学习过程，从而可能影响蜜蜂觅食效率。这些结果的影响进行了讨论的风险转基因Bt作物的蜜蜂。
Genetically modified Bt crops are increasingly used worldwide but side effects and especially sublethal effects on beneficial insects remain poorly studied. Honey bees are beneficial insects for natural and cultivated ecosystems through pollination. The goal of the present study was to assess potential effects of two concentrations of Cry1Ab protein (3 and 5000ppb) on young adult honey bees. Following a complementary bioassay, our experiments evaluated effects of the Cry1Ab on three major life traits of young adult honey bees: (a) survival of honey bees during sub-chronic exposure to Cry1Ab, (b) feeding behaviour, and (c) learning performance at the time that honey bees become foragers. The latter effect was tested using the proboscis extension reflex (PER) procedure. The same effects were also tested using a chemical pesticide, imidacloprid, as positive reference. The tested concentrations of Cry1Ab protein did not cause lethal effects on honey bees. However, honey bee feeding behaviour was affected when exposed to the highest concentration of Cry1Ab protein, with honey bees taking longer to imbibe the contaminated syrup. Moreover, honey bees exposed to 5000ppb of Cry1Ab had disturbed learning performances. Honey bees continued to respond to a conditioned odour even in the absence of a food reward. Our results show that transgenic crops expressing Cry1Ab protein at 5000ppb may affect food consumption or learning processes and thereby may impact honey bee foraging efficiency. The implications of these results are discussed in terms of risks of transgenic Bt crops for honey bees.