The expression of human relaxin in yeast.
The expression of human relaxin in yeast.
复制标题
人松弛素在酵母中的表达。
DOI：
10.1006/abbi.1993.1101
复制
发表时间：
1993
影响因子：
3.9
通讯作者：
Schwabe,C
中科院分区：
文献类型：
--
作者：
Yang,S;Heyn,H;Zhang,YZ;Bullesbach,EE;Schwabe,C
利用化学合成的人工单链人松弛素dna编码序列，构建了两个质粒，分别导入酿酒酵母（saccharomyces cerevisiae）。该dna编码序列由一条B链、一条arg - arg - glu - ph - lys - arg连接肽和一条a链组成。松弛素编码序列的表达受酵母TDH3启动子或CUP1启动子的控制。利用酵母α-因子信号序列引导蛋白进入分泌途径，通过放射免疫法和免疫印迹法证实生长培养基中出现人松弛素。从酵母中部分纯化的人松弛素在小鼠耻骨联合试验和放射受体试验中具有生物活性。
The chemically synthesized DNA-coding sequence for an artificial single chain human relaxin consisting of a B chain, an Arg-Arg-Glu-Phe-Lys-Arg-connecting peptide, followed by the A chain, was used to construct two plasmids which were introduced intoSaccharomyces cerevisiae. Expression of the relaxin-coding sequence was under the control of either the yeast TDH3 promoter or the CUP1 promoter. The yeast α-factor signal sequence was used to direct the protein into the secretory path, and the appearance of human relaxin in the growth medium was confirmed by radioimmunoassay and immunoblotting. Partially purified human relaxin from yeast was biologically active in the mouse symphysis pubis assay and radioreceptor assay.