DAPI AS A USEFUL STAIN FOR NUCLEAR QUANTITATION
DAPI AS A USEFUL STAIN FOR NUCLEAR QUANTITATION
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DOI：
10.3109/10520299109109990
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发表时间：
1991-01-01
影响因子：
1.6
通讯作者：
NICHOLSON, JH
中科院分区：
文献类型：
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作者：
TARNOWSKI, BI;SPINALE, FG;NICHOLSON, JH
4', 6-二脒基-2-苯基吲哚 (DAPI) 是一种简单易用的荧光染料，可显示活细胞和固定细胞中的核 DNA。 DAPI 染色用于测定细胞核数量并评估大体细胞形态。光学显微镜分析后，将染色的细胞进行电子显微镜处理。与未用 DAPI 染色的细胞的外观相比，用 DAPI 染色的细胞未显示超微结构变化。 DAPI 染色允许多次使用细胞，无需重复样品。
A simple-to-use fluorescent stain, 4', 6-diamidino-2-phenylindole (DAPI), visualizes nuclear DNA in both living and fixed cells. DAPI staining was used to determine the number of nuclei and to assess gross cell morphology. Following light microscopic analyses, the stained cells were processed for electron microscopy. Cells stained with DAPI showed no ultrastructural changes compared to the appearance of cells not stained with DAPI. DAPI staining allows multiple use of cells eliminating the need for duplicate samples.