Serum metabolomics study in a group of Parkinson's disease patients from northern India
Serum metabolomics study in a group of Parkinson's disease patients from northern India
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DOI：
10.1016/j.cca.2018.02.022
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发表时间：
2018-05-01
影响因子：
5
通讯作者：
Roy, Etaja
中科院分区：
文献类型：
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作者：
Babu, G. Nagesh;Gupta, Manjeet;Roy, Etaja
背景：帕金森病 (PD) 是黑质纹状体多巴胺能通路进行性退化和纹状体中神经递质多巴胺耗竭的结果。方法：我们纳入了 17 名帕金森病患者、7 名进行性核上性麻痹 (PSP) 患者、6 名多系统萎缩 (MSA) 患者以及 22 名年龄和性别匹配的健康对照者。我们使用 H-1 NMR 光谱分析了这些患者和对照组血清中的代谢物谱。结果：PD、PSP 和 MSA 中的异亮氨酸、缬氨酸、丙氨酸、谷氨酰胺和组氨酸显着（P < 0.001）高于对照组，而 PD（P < 0.001）、PSP 和 MSA 中谷氨酸和葡萄糖显着增加（P < 0.05） 与控制。与对照相比，PD、PSP 和 MSA 中的柠檬酸盐增加 (P < 0.05)。丙酮、乳酸和甲酸含量较高，P < 0.001，苏氨酸含量较高，P < 0.05。 OPLS-DA 模型的 3D 散点评分图显示各组之间存在明显差异，R2 = 0.92，Q2 = 0.78。结论：对照组和疾病组之间各种代谢物水平存在显着差异。所有组中显着较高的常见氨基酸包括支链氨基酸，这可以增加神经元的兴奋性。
Background: Parkinson's disease (PD) is the result of progressive degeneration of the nigrostriatal dopaminergic pathway and depletion of neurotransmitter dopamine in the striatum.Methods: We included 17 patients with PD along with 7 patients of progressive supranuclear palsy (PSP), 6 patients of multiple system atrophy (MSA) and 22 age and sex-matched healthy controls. We analyzed metabolite profiles in the serum of these patients and controls using H-1 NMR spectroscopy.Results: Isoleucine, valine, alanine, glutamine and histidine in PD, PSP and MSA were significantly (P < 0.001) higher than controls, whereas, glutamate and glucose were significantly increased in PD (P < 0.001), PSP and MSA (P < 0.05) vs. control. Citrate was increased in PD, PSP and MSA (P < 0.05) vs. control. While, acetone, lactate and formate were higher at P < 0.001, threonine is increased at P < 0.05. The 3D scattered score plot of OPLS-DA model revealed clear differentiation among the groups, R2 = 0.92 and Q2 = 0.78.Conclusion: Significant differences in various metabolite levels were found between control and disease groups. Common amino acids that are significantly higher in all groups include branched chain amino acids, which could increase neuronal excitability.