Construction of a protein-detection system using a loop peptide library with a fluorescence label.
Construction of a protein-detection system using a loop peptide library with a fluorescence label.
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DOI：
10.1016/s1074-5521(02)00308-3
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发表时间：
2003
影响因子：
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通讯作者：
Mizuki Takahashi;K. Nokihara;H. Mihara
中科院分区：
文献类型：
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作者：
Mizuki Takahashi;K. Nokihara;H. Mihara
设计了一种基于环状结构的荧光标记肽库，构建了一种新型的蛋白质检测系统。作为基本模型研究，检查了使用源自tendamistat活性环的荧光标记肽检测α-淀粉酶。成功地建立了固定化多肽和溶液中多肽对蛋白质的检测方法。基于这些结果，已经构建了具有接枝在稳定环结构上的各种转角序列的环肽文库。具有对应于例如“蛋白质指纹”的识别模式的各种蛋白质可以使用固定化肽文库来检测。目前的结果表明，该系统可以应用于肽微阵列的发展，表现为一个蛋白质芯片。
Construction of a novel protein-detection system was carried out using a designed peptide library with fluorescent labels based on loop structures. As a basic model study, detection of α-amylase using fluorescent-labeled peptides derived from an active loop of tendamistat was examined. The detection methods for proteins with immobilized peptides as well as peptides in solution have been successfully established. Based on these results, a loop peptide library that has various turn sequences grafted on a stable loop structure has been constructed. Various proteins with recognition patterns corresponding, for instance, to "protein fingerprints" could be detected using an immobilized peptide library. The present results suggest that the system can be applied to the development of a peptide microarray that behaves as a protein chip.