Exopolysaccharides from Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 prevent influenza virus infection and attenuate secondary bacterial infection risk
Exopolysaccharides from Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 prevent influenza virus infection and attenuate secondary bacterial infection risk
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DOI：
10.1111/lam.13649
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发表时间：
2022-01-26
影响因子：
2.4
通讯作者：
Iyoda, M.
中科院分区：
文献类型：
--
作者：
Ishikawa, H.;Kuno, Y.;Iyoda, M.
本文研究了德布鲁克氏乳杆菌（Lactobacillus delbrueckii ssp）对胞外多糖（EPS）的抑制作用。保加利亚菌OLL1073R-1抗流感病毒感染继发细菌感染。我们发现，当A549细胞在感染前被EPS处理，而在感染后不被EPS处理时，200或400 μ g ml（-1）的EPS对流感病毒感染有显著的保护作用，并呈剂量依赖性。与未感染流感病毒的A549细胞相比，感染流感病毒的A549细胞上癌胚抗原相关细胞粘附分子1 （CEACAM-1）的表达显著增强。然而，在病毒感染前，EPS处理显著降低了这种上调的CEACAM-1表达，这与A549细胞中病毒滴度的降低有关。用金黄色葡萄球菌进行细菌黏附实验，发现细菌对病毒感染的A549细胞的黏附明显大于对未感染病毒的A549细胞的黏附，并且在感染前EPS处理有降低流感病毒感染引起的细菌黏附的趋势。我们的研究结果表明，在病毒感染前治疗EPS可以通过降低CEACAM-1的表达来抑制流感病毒感染并减轻继发性细菌感染。
The present study assessed the inhibitory action of exopolysaccharides (EPS) produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 against influenza virus infection followed by secondary bacterial infection. We found that the presence of 200 or 400 mu g ml(-1) of EPS significantly protected against influenza virus infection in a dose-dependent manner when A549 cells were treated with EPS before infection but not after it. The expression of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1), an adhesion molecule for bacteria adherence, on A549 cells was significantly enhanced during influenza virus infection compared with viral-non-infected A549 cells. However, this upregulated CEACAM-1 expression was significantly decreased by EPS treatment before viral infection in association with the reduction in the virus titre in A549 cells. In a bacterial adhesion assay using Staphylococcus aureus, the bacterial adherence to viral-infected A549 cells was significantly greater than that to viral-non-infected A549 cells, and the increased bacterial adherence induced by influenza virus infection tended to be decreased by EPS treatment before the infection. Our findings show that EPS treatment before viral infection can inhibit influenza virus infection and alleviate secondary bacterial infection through decreased CEACAM-1 expression.