Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas
Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas
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DOI：
10.1126/science.1228638
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发表时间：
2012-10-26
期刊：
影响因子：
56.9
通讯作者：
Tinnefeld, P.
中科院分区：
文献类型：
--
作者：
Acuna, G. P.;Moeller, F. M.;Tinnefeld, P.
我们引入自组装的纳米天线，以提高荧光强度的等离子热点的zeptoliter体积。纳米天线是通过将一个或两个金纳米颗粒（NP）附着到DNA折纸结构上来制备的，DNA折纸结构还在一个NP旁边或两个NP之间的差距中掺入了单个荧光染料的对接位点。我们测量了NP的大小和数量上的荧光增强的依赖性，并将其与数值模拟进行比较。对于位于100纳米金纳米颗粒之间的23纳米间隙中的染料分子，获得了最大117倍的荧光增强。短DNA链的结合和解结合的直接可视化，以及在纳米天线的热点中的DNA霍利迪连接的构象动力学，显示与单分子测定的兼容性。
We introduce self-assembled nanoantennas to enhance the fluorescence intensity in a plasmonic hotspot of zeptoliter volume. The nanoantennas are prepared by attaching one or two gold nanoparticles (NPs) to DNA origami structures, which also incorporated docking sites for a single fluorescent dye next to one NP or in the gap between two NPs. We measured the dependence of the fluorescence enhancement on NP size and number and compare it to numerical simulations. A maximum of 117-fold fluorescence enhancement was obtained for a dye molecule positioned in the 23-nanometer gap between 100-nanometer gold NPs. Direct visualization of the binding and unbinding of short DNA strands, as well as the conformational dynamics of a DNA Holliday junction in the hotspot of the nanoantenna, show the compatibility with single-molecule assays.