Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis
Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis
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DOI：
10.1242/dev.00843
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Baylies, M
中科院分区：
文献类型：
--
作者：
Artero, R;Furlong, EE;Baylies, M
果蝇肌肉起源于两种类型的成肌细胞的融合，创始人细胞（FC）和融合感受态成肌细胞（FCM）。为了更好地了解肌肉的多样性和形态发生，我们进行了大规模的基因表达分析，以确定在FC和FCM中差异表达的基因。我们采用来自Toll（10b）突变体的胚胎以获得主要形成肌肉的中胚层，并表达Ras或Notch的活化形式以分别诱导FC或FCM命运。通过与cDNA微阵列杂交来比较每个基因型胚胎中存在的转录物。在83个差异表达的基因中，我们发现了已知在FC或FCM中富集的基因，如无情或hibris，先前在肌肉发育中具有未知作用的特征基因，以及未知功能的预测基因。我们对新发现的基因的研究揭示了基因表达的新模式，仅限于两种类型的成肌细胞之一，也是引人注目的肌肉表型。虽然基因，如叶状足类在特定肌肉的规范中起着至关重要的作用，但其他基因，如格子呢，对于正常的肌肉形态发生是必要的。
Drosophila muscles originate from the fusion of two types of myoblasts, founder cells (FCs) and fusion-competent myoblasts (FCMs). To better understand muscle diversity and morphogenesis, we performed a large-scale gene expression analysis to identify genes differentially expressed in FCs and FCMs. We employed embryos derived from Toll(10b) mutants to obtain primarily muscle-forming mesoderm, and expressed activated forms of Ras or Notch to induce FC or FCM fate, respectively. The transcripts present in embryos of each genotype were compared by hybridization to cDNA microarrays. Among the 83 genes differentially expressed, we found genes known to be enriched in FCs or FCMs, such as heartless or hibris, previously characterized genes with unknown roles in muscle development, and predicted genes of unknown function. Our studies of newly identified genes revealed new patterns of gene expression restricted to one of the two types of myoblasts, and also striking muscle phenotypes. Whereas genes such as phyllopod play a crucial role during specification of particular muscles, others such as tartan are necessary for normal muscle morphogenesis.