Bacterial population moves toward a colon-like community in the pouch after total proctocolectomy
Bacterial population moves toward a colon-like community in the pouch after total proctocolectomy
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DOI：
10.1016/j.surg.2008.12.003
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发表时间：
2009-04-01
期刊：
影响因子：
3.8
通讯作者：
Fukushima, Kouhei
中科院分区：
文献类型：
--
作者：
Kohyama, Atsushi;Ogawa, Hitoshi;Fukushima, Kouhei
背景。结肠转化是指全直肠结肠切除术后回肠的表型改变。然而，回肠袋微生物群的变化以及这些微生物在结肠转化中的作用尚未得到解决。方法。共采集151份粪便样本，采集者为溃疡性结肠炎肛门回肠造口患者、回肠袋患者和健康对照志愿者。从粪便中提取细菌 DNA，并通过末端限制性片段长度多态性 (T-RFLP) 分析评估复杂细菌的多样性，这是一种基于 DNA 的新型方法，使我们能够研究不可培养细菌的存在。 75 确定回肠贮袋细菌群落是否转移到a。更像结肠的分布，末端限制性的相对丰度，可以分类为“结肠”、“回肠”或“常见”的片段。进行了调查。结果。聚类分析表明，大多数回肠造口样本被归类为聚类 I 或 II，不到 10% 的回肠造口样本被归类为聚类 IV。在。相比之下，超过 90% 的对照样品被分组在簇 IV 中。在进一步分析中，簇 I、II、III 和 IV 中的造口袋的中位寿命分别显着不同，分别为 11、56、265 和 310 天。回肠袋的T-RFLP模式的特征在于“回肠”随时间的减少和部分“结肠”片段的增加，这些片段主要代表不可培养的细菌，例如球状梭菌群。结论。 T-RFLP 分析表明，全直肠结肠切除术后回肠袋中出现“结肠样”细菌群落（包括不可培养细菌）的时间依赖性转变。 （外科手术 2009；145：435-47。）
Background. Colonic transformation is defined by phenotypic alterations in, the ileum after total proctocoleetomy. Changes in microbiota of the ileal pouch and the roles of these microbes in colonic transformation, however, have not, been addressed.Methods. A total of 151 stool samples were collected front patients with ulcerative colitis patients anal an ileostomy, those with an ileal pouch, and healthy control volunteers. Bacterial DNA was extracted from stool, and the diversity of complex bacteria was assessed by terminal restriction fragment length polymorphism (T-RFLP) analysis, a novel DNA-based approach that enables us to investigate the presence of nonculturable bacteria. 75 determine whether ileal pouch bacterial communities shift to a. more colon-like distribution, the relative abundance of terminal restriction, fragments that could be classified, as "colonic," "ileal," or "common." was investigated.Results. Cluster analysis demonstrated that most of the ileostomy samples were categorized into Cluster I or II and that less than 10% of ileostomy samples were classified into Cluster IV. In. contrast, more than 90% of control samples were grouped in Cluster IV In further analyses, the median lifetimes of pouches in Clusters I, II, III, and IV were significantly different at 11, 56, 265, and 310 days, respectively. T-RFLP patterns of the ileal pouch were characterized, by a time-dependent decrease in "ileal" and increase in a part of "colonic" fragments, which represented mainly nonculturable bacteria such as the Clostridium coccoides group.Conclusion. T-RFLP analysis demonstrated that a time-dependent shift, to a "colon-like" bacterial, community, including nonculturable bacteria, in the ileal pouch after total proctocolectomy. (Surgery 2009;145:435-47.)