Morphological difference of Escherichia coli non-heme ferritin iron cores reconstituted in the presence and absence of inorganic phosphate
Morphological difference of Escherichia coli non-heme ferritin iron cores reconstituted in the presence and absence of inorganic phosphate
复制标题
存在和不存在无机磷酸盐时重构的大肠杆菌非血红素铁蛋白铁核的形态差异
DOI：
10.1007/s00775-022-01952-5
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Ikeguchi Masamichi
中科院分区：
文献类型：
--
作者：
Kuwata Takumi;Sato Daisuke;Yanagida Yuki;Aoki Eriko;Fujiwara Kazuo;Yoshimura Hideyuki;Ikeguchi Masamichi
大肠杆菌的铁核心ofEscherichia coliferritin重建在存在和不存在的磷酸盐。在磷酸盐存在下形成的核含有与铁含量相当的量的磷酸盐。通过分析超离心法分析了岩心的粒度分布。在磷酸盐存在下观察到连续的尺寸分布，而在不存在磷酸盐的情况下发现多峰分布。在磷酸盐存在的情况下，电子显微镜观察到的核心尺寸与铁蛋白的内径一致。与此相反，在不存在磷酸盐的情况下观察到了几个较小颗粒的聚集。在对比度匹配条件下测量小角X射线散射，以获得关于铁芯形状的信息。在存在磷酸盐的情况下，在散射轮廓中观察到条纹，但在不存在磷酸盐的情况下没有观察到条纹。结合所有结果，我们得出结论，在磷酸盐存在下形成中空球形核心，而在不存在磷酸盐的情况下，在内腔中形成几个小颗粒。图形摘要
The iron core ofEscherichia coliferritin was reconstituted in the presence and absence of phosphate. The core formed in the presence of phosphate contained phosphate in amounts comparable to the iron content. The size distribution of the core was analyzed by analytical ultracentrifugation. A continuous size distribution was observed in the presence of phosphate, whereas a multimodal distribution was found in the absence of phosphate. In the presence of phosphate, the core size observed by electron microscopy was consistent with the inner diameter of ferritin. In contrast to this, clusters of several smaller particles were observed in the absence of phosphate. The small-angle X-ray scattering was measured under contrast matching conditions to obtain information on the iron core shape. A fringe was observed in the scattering profile in the presence of phosphate, but it was not observed in the absence of phosphate. Combining all results, we conclude that a hollow spherical core was formed in the presence of phosphate, while several small particles were formed within the inner cavity in the absence of phosphate.Graphical abstract