ELECTRON MICROSCOPIC STUDIES ON THE BLOOD‐AQUEOUS BARRIER OF PROSTAGLANDIN‐TREATED RABBIT EYES
ELECTRON MICROSCOPIC STUDIES ON THE BLOOD‐AQUEOUS BARRIER OF PROSTAGLANDIN‐TREATED RABBIT EYES
复制标题
前列腺素处理兔眼血水屏障的电子显微镜研究
作者：
O. Pedersen
将前列腺素E1和E2局部应用于兔眼。用电子显微镜研究了虹膜和睫状突中与血-房水屏障相关的结构，以及睫状上皮对蛋白示踪剂辣根过氧化物酶的渗透性。在虹膜突上皮中发现了显著的形态学变化，包括细胞间隙的扩张和两个上皮细胞层的分离。睫状突上皮仅发现微小的结构变化。在虹膜突和睫状突的前部，过氧化物酶通过上皮细胞间隙的泄漏被证明。在睫状血管的同一地区，开放的内皮间隙，血小板聚集，微血栓，和出血被found. In以前的体外研究的影响，野牡丹素的运动的过氧化物酶在睫状上皮，没有发现上皮结构的变化，和上皮扩散屏障过氧化物酶被发现是完整的。据推测，这种屏障在体内的破坏是继发于血管变化。
Prostaglandins E1 and E2 were applied topically to rabbit eyes. Structures related to the blood‐aqueous barrier in the iridial and ciliary processes, as well as the permeability of the ciliary epithelium to the protein tracer horseradish peroxidase, were studied with the electron microscope. Marked morphological changes, including dilations of the intercellular spaces and separation of the two epithelial cell layers, were found in the epithelium of the iridial processes. Only minor structural changes were found in the epithelium of the ciliary processes. Leakage of peroxidase through the intercellular spaces of the epithelium was demonstrated in the iridial processes and in the anterior parts of the ciliary processes. In the ciliary vessels of the same regions, opening of interendothelial gaps, platelet aggregations, microthrombi, and haemorrhages were found. In a previous in vitro study on the effects of prostaglandins on the movement of peroxidase in the ciliary epithelium, no structural changes of the epithelium were found, and the epithelial diffusion barrier to peroxidase was found to be intact. It is assumed that the breakdown of this barrier in vivo is secondary to vascular changes.