Loss of Thy-1 (CD90) antigen expression on mesenchymal stromal cells from hematologic malignancies is induced by in vitro angiogenic stimuli and is associated with peculiar functional and phenotypic characteristics
Loss of Thy-1 (CD90) antigen expression on mesenchymal stromal cells from hematologic malignancies is induced by in vitro angiogenic stimuli and is associated with peculiar functional and phenotypic characteristics
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DOI：
10.1080/14653240701762364
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发表时间：
2008-01-01
期刊：
影响因子：
4.5
通讯作者：
Cuneo, A.
中科院分区：
文献类型：
--
作者：
Campioni, D.;Lanza, F.;Cuneo, A.
背景人类间充质基质细胞（hMSC）的表型和功能亚群对环境刺激的反应知之甚少。在这项研究中使用的策略集中在定义hMSC功能亚群的基础上，特别是他们的Thy-1（CD 90）抗原（Ag）的表面expression.MethodsThe不同的体外微环境条件对骨髓来源的（BM）hMSC的分离和扩增血液恶性肿瘤（HM）和正常样本（NS）的影响进行了测定。hMSC克隆和分化潜力，表型和长期能力，以维持在体外造血被认为是在不同的expansion protocols.ResultsThe结果表明，血管生成补充剂结合使用低血清含量引起的外观的Thy-1-HM-MSC具有高增殖潜力，能够恢复典型的HM基质损害。CD 271的表达得到部分维持。我们进一步报道了Thy-1- HM-MSC亚群对成骨和成脂分化能力的增强。尽管血管生成治疗，Thy-1- MSC停止短的完全endothelial differentiation.DiscussionIn本文中，我们提供的证据表明，在体外血管生成刺激生成HM-MSC缺乏CD 90 Ag表达。Thy-1- MSC亚群的特征在于独特的功能和表型特征，从而支持微环境在选择维持正常组织稳态或诱导病理过程的特定hMSC亚群中所发挥的作用。
BackgroundLittle is known about human mesenchymal stromal cell (hMSC) phenotypic and functional subsets in response to environmental stimuli. The strategy used in this study focused on defining hMSC functional subpopulations based in particular on their Thy-1 (CD90) antigen (Ag) surface expression.MethodsThe effect of different in vitro microenvironmental conditions on the isolation and expansion of bone marrow-derived (BM) hMSC from hematologic malignancies (HM) and normal samples (NS) was assayed. hMSC clonogenic and differentiation potential, phenotypic profile and long-term capacity to sustain in vitro hemopoiesis were considered in relation to the different expansion protocols.ResultsThe results showed that angiogenic supplements used in combination with low serum content gave rise to the appearance of Thy-1- HM-MSC with high proliferative potential, capable of restoring the typical HM stromal impairment. The expression of the CD271 was partially maintained. We further report an enhancement towards the osteogenic and adipogenic differentiation capacity by the Thy-1- HM-MSC subset. Despite the angiogenic treatment, the Thy-1- MSC stopped short of full endothelial differentiation.DiscussionIn this paper we provide evidence that in vitro angiogenic stimuli generate HM-MSC lacking CD90 Ag expression. The Thy-1- MSC subset is characterized by peculiar functional and phenotypic characteristics, thus supporting the role played by the microenvironment in selecting particular hMSC subsets maintaining normal tissue homeostasis or inducing pathologic processes.