Preparation and characterization of human gingival cells.
Preparation and characterization of human gingival cells.
复制标题
人牙龈细胞的制备和表征。
DOI：
10.1111/j.1600-0765.1987.tb01554.x
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发表时间：
1987
影响因子：
3.5
通讯作者：
Smith,DJ
中科院分区：
文献类型：
--
作者：
Stoufi,ED;Taubman,MA;Ebersole,JL;Smith,DJ
开发了一种程序，用于使用胶原酶消化然后温和机械破碎从牙龈和牙周组织制备单细胞悬液。在大鼠牙龈组织上评价该程序。发现孵育1小时产生的细胞数量最多，活力最高，单核细胞回收率最高。胶原酶似乎不影响人外周血细胞的回收率、活力或B细胞表面标志物。人牙龈和牙周组织来自35例患者：20例成人牙周炎，8例青少年牙周炎，7例正常牙龈炎或慢性牙龈炎。常规进行刮治，然后进行探查测量，然后对受影响部位进行手术。在从手术获得的组织的单细胞悬液中，从正常/慢性牙龈炎患者的组织中回收的单核细胞显著少于从成人牙周炎或青少年牙周炎组回收的细胞/mg组织。血液单核细胞对牙龈细胞的最大贡献平均为0.5 ± 0.2%。该方法可用于回收牙龈（牙周）细胞用于表型和功能研究。
A procedure was developed for the preparation of single cell suspensions from gingival and periodontal tissues using collagenase digestion followed by gentle mechanical disruption. The procedure was evaluated on rat gingival tissues. One‐hour incubation was found to produce the greatest number of cells with the highest viability, and the largest recovery of mononuclear cells. Collagenase did not appear to effect recovery, viability or B cell surface markers on human peripheral blood cells. Human gingival and periodontal tissues were obtained from 35 patients: 20 with adult periodontitis; 8 with juvenile periodontitis; 7 with normal gingivae or chronic gingivitis. Scaling, followed by probing measurements and then surgery of the affected sites was routinely performed. In single cell suspensions from tissue obtained from surgery, significantly fewer mononuclear cells were recovered from the tissues of normal/chronic gingivitis patients than cells/mg of tissue recovered from adult periodontitis or juvenile periodontitis groups. The maximum contribution of blood mononuclear cells to the gingival cells averaged 0.5+0.2%. This method is useful for recovering gingival (periodontal) cells for phenotypic and functional studies.