Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy.
Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy.
复制标题
使用单曝光倾斜背照式显微镜对微循环进行视频速率成像。
DOI：
10.1117/1.jbo.18.6.066007
复制
发表时间：
2013
影响因子：
3.5
通讯作者：
Mertz,Jerome
中科院分区：
文献类型：
--
作者：
Ford,TimN;Mertz,Jerome
斜背照明显微术（OBM）是一种基于宽视场成像的厚散射组织中相位梯度和吸收的同时、独立测量的新技术。到目前为止，OBM已经与顺序相机曝光一起使用，这降低了时间分辨率，并且可以在动态样本中产生运动伪影。在这里，OBM的变化，允许单次曝光操作与波长复用和图像分裂与沃拉斯顿棱镜介绍。利用图形处理单元对棱镜引起的非对称畸变进行真实的实时表征和校正。为了证明单次曝光OBM执行无伪影血流成像的能力，提供了发育中的鸡的绒毛尿囊膜中的卵内微循环的视频速率电影。成像采用适用于内窥镜检查的高分辨率刚性霍普金斯透镜进行。
Oblique back-illumination microscopy (OBM) is a new technique for simultaneous, independent measurements of phase gradients and absorption in thick scattering tissues based on widefield imaging. To date, OBM has been used with sequential camera exposures, which reduces temporal resolution, and can produce motion artifacts in dynamic samples. Here, a variation of OBM that allows single-exposure operation with wavelength multiplexing and image splitting with a Wollaston prism is introduced. Asymmetric anamorphic distortion induced by the prism is characterized and corrected in real time using a graphics-processing unit. To demonstrate the capacity of single-exposure OBM to perform artifact-free imaging of blood flow, video-rate movies of microcirculationin ovoin the chorioallantoic membrane of the developing chick are presented. Imaging is performed with a high-resolution rigid Hopkins lens suitable for endoscopy.