Immunolocalization of angiogenic growth factors in the ovine uterus during the oestrus cycle and in response to Steroids.
Immunolocalization of angiogenic growth factors in the ovine uterus during the oestrus cycle and in response to Steroids.
复制标题
发情周期期间绵羊子宫中血管生成生长因子的免疫定位以及对类固醇的反应。
DOI：
10.1111/rda.13156
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Tremaine TD
中科院分区：
文献类型：
--
作者：
Tremaine TD
内容与胚胎着床前子宫内膜成熟相关的血管变化依赖于多种生长因子，已知这些生长因子调节关键的血管生成事件。首先，血管内皮生长因子（VEGF）家族促进血管生长，而血管生成素维持血管完整性。目的是分析发情周期卵泡期和黄体期绵羊子宫内膜中VEGFA配体和受体、血管生成素-1和2（ANG 1/2）和内皮细胞受体酪氨酸激酶（TIE-2）的蛋白水平以及对卵巢类固醇的反应。VEGFA及其受体定位于血管细胞和非血管上皮（腺上皮和腔上皮）以及基质细胞中。VEGFA和VEGFR 2蛋白在卵泡期子宫内膜的血管细胞中升高，与黄体期相比，对雌二醇的反应最显着。VEGFR 1由上皮细胞和内皮细胞表达，并在雌二醇刺激下表达。相比之下，与卵泡期相比，黄体期子宫内膜中的Ang-1和Ang-2蛋白升高，并且对孕酮有反应，这在血管平滑肌细胞和TIE-2表达血管周围的腺体中很明显。我们的研究结果表明，VEGFA受雌二醇刺激，最主要是在卵泡期子宫内膜中，而Ang-1和2受孕酮刺激，并在发情周期的黄体期，血管成熟期间增加。
ContentsThe vascular changes associated with endometrial maturation in preparation for embryo implantation depend on numerous growth factors, known to regulate key angiogenic events. Primarily, the vascular endothelial growth factor (VEGF) family promotes vascular growth, whilst the angiopoietins maintain blood vessel integrity. The aim was to analyse protein levels of VEGFA ligand and receptors, Angiopoietin‐1 and 2 (ANG1/2) and endothelial cell receptor tyrosine kinase (TIE‐2) in the ovine endometrium in the follicular and luteal phases of the oestrus cycle and in response to ovarian steroids. VEGFA and its receptors were localized in both vascular cells and non‐vascular epithelium (glandular and luminal epithelium) and stroma cells. VEGFA and VEGFR2 proteins were elevated in vascular cells in follicular phase endometrium, compared to luteal phase, most significantly in response to oestradiol. VEGFR1 was expressed by epithelial cells and endothelial cells and was stimulated in response to oestradiol. In contrast, Ang‐1 and Ang‐2 proteins were elevated in luteal phase endometrium compared to follicular phase, and in response to progesterone, evident in vascular smooth muscle cells and glands which surround TIE‐2‐expressing blood vessels. Our findings indicate that VEGFA is stimulated by oestradiol, most predominantly in follicular phase endometrium, and Ang‐1 and 2 are stimulated by progesterone and were increased during the luteal phase of the oestrus cycle, during the time of vascular maturation.