Estimating a suitable microsatellite marker set for individual identification and parentage tests of brown bear (Ursus arctos) in the Akan–Shiranuka Region, eastern Hokkaido, Japan
Estimating a suitable microsatellite marker set for individual identification and parentage tests of brown bear (Ursus arctos) in the Akan–Shiranuka Region, eastern Hokkaido, Japan
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DOI：
10.1007/s10310-009-0110-3
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发表时间：
2009-02
影响因子：
1.5
通讯作者：
T. Itoh;Yoshikazu Sato;T. Mano;R. Iwata
中科院分区：
文献类型：
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作者：
T. Itoh;Yoshikazu Sato;T. Mano;R. Iwata
我们研究了24个微卫星标记，以选择最适合的个体识别和亲子鉴定的棕熊（熊）的基础上的统计参数和实验误差，使用肝脏样本（38个问题）的滋扰熊杀死在阿寒，白熊地区，东北海道，日本从1996年至2006年。我们发现了7个适合识别个体的微卫星标记（G1 A、G10 B、G10 L、UarMU 5、UarMU 23、UarMU 50和UarMU 51;同一性概率Pid = 3.17 × 10 - 7;同一性概率 sibsPid-sib= 2.23 × 10−3），12个微卫星 当父母中有一方 已知[G1 A，G1 D，G10 B，G10 L，G10 P，UarMU 5，UarMU 23，UarMU 50，UarMU 51， UarMU 59、UarMU 61和UarMU 64;排除一个亲本的概率 (PE I = 0.9991）]和15个微卫星标记 当父母都不知道的时候， 以上：G10 M、G10 X和UarMU 9; 排除双亲的概率（PE II = 0.9869）]。
We examined 24 microsatellite markers to select those most suitable for individual identification and parentage tests of brown bears (Ursus arctos) based on statistical parameters and experimental error using liver samples (38 issues) from nuisance bears killed in the Akan–Shiranuka Region, eastern Hokkaido, Japan from 1996 to 2006. We found seven microsatellite markers suitable for identifying individuals (G1A, G10B, G10L, UarMU5, UarMU23, UarMU50, and UarMU51; probability of identityPid= 3.17 × 10−7; probability of identity of sibsPid-sib= 2.23 × 10−3), 12 microsatellite markers suitable for parentage testing when one parent is known [G1A, G1D, G10B, G10L, G10P, UarMU5, UarMU23, UarMU50, UarMU51, UarMU59, UarMU61, and UarMU64; probability of excluding one parent (PE I = 0.9991)], and 15 microsatellite markers for parentage testing when neither parents are known [three markers added to the above: G10M, G10X, and UarMU9; probability of excluding both parents (PE II = 0.9869)].