Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content
Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content
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DOI：
10.1007/bf00222202
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发表时间：
1995-02
影响因子：
5.4
通讯作者：
M. Uzunova;Wolfgang Ecke;K. Weissleder;G. Robbelen
中科院分区：
文献类型：
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作者：
M. Uzunova;Wolfgang Ecke;K. Weissleder;G. Robbelen
以甘蓝型油菜品种‘Mansholt’Hamburger Raps‘和’Samourai‘杂交F_1代的加倍单倍体群体为材料，构建了一个由204个RFLP标记、2个RAPD标记和1个表型标记组成的油菜基因组连锁图谱。所定位的标记分布在19个连锁群上，覆盖1441 cM。这些标记中约有43%是显性的，36%的标记座位是重复的，保守的连锁排列表明油菜基因组中有重复的区域。有27.8%的标记偏离孟德尔分离比。这些标记大多聚集在7个连锁群上的7个大区块中，表明造成偏向分离的有效因素数量相等。利用酰基载体蛋白(ACP)和β-酮酰基-ACP-合成酶I(KASI)基因的c DNA探针，分别定位了3个和2个基因座。利用连锁图谱对种子硫代葡萄糖苷含量的QTL进行区间定位。四个QTL可以定位在四个连锁群上，给出了参与该性状遗传控制的最小数量的因素。所定位的QTL的估计效应解释了两个亲本之间差异的约74%，解释了在加倍单倍体定位群体中观察到的约61.7%的表型变异。
A linkage map of the rapeseed genome comprising 204 RFLP markers, 2 RAPD markers, and 1 phenotypic marker was constructed using a F1derived doubled haploid population obtained from a cross between the winter rapeseed varieties ‘Mansholt's Hamburger Raps’ and ‘Samourai’. The mapped markers were distributed on 19 linkage groups covering 1441 cM. About 43% of these markers proved to be of dominant nature; 36% of the mapped marker loci were duplicated, and conserved linkage arrangements indicated duplicated regions in the rapeseed genome. Deviation from Mendelian segregation ratios was observed for 27.8% of the markers. Most of these markers were clustered in 7 large blocks on 7 linkage groups, indicating an equal number of effective factors responsible for the skewed segregations. Using cDNA probes for the genes of acyl-carrier-protein (ACP) andβ-ketoacyl-ACP-synthase I (KASI) we were able to map three and two loci, respectively, for these genes. The linkage map was used to localize QTLs for seed glucosinolate content by interval mapping. Four QTLs could be mapped on four linkage groups, giving a minimum number of factors involved in the genetic control of this trait. The estimated effects of the mapped QTLs explain about 74% of the difference between both parental lines and about 61.7 % of the phenotypic variance observed in the doubled haploid mapping population.