Metabolic imaging by hyperpolarized 13C magnetic resonance imaging for in vivo tumor diagnosis
Metabolic imaging by hyperpolarized 13C magnetic resonance imaging for in vivo tumor diagnosis
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DOI：
10.1158/0008-5472.can-06-2564
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发表时间：
2006-11-15
期刊：
影响因子：
11.2
通讯作者：
Ardenkjaer-Larsen, Jan Henrik
中科院分区：
文献类型：
--
作者：
Golman, Klaes;in't Zandt, Rene;Ardenkjaer-Larsen, Jan Henrik
“Warburg效应”，即有氧糖酵解的升高，可能是癌细胞的一个基本特性。对于癌症的诊断和治疗，如果能在动物和人类的图像中量化糖酵解代谢的升高，将是有价值的。丙酮酸分子处于细胞内能量传递的代谢十字路口，通过对丙酮酸在生物学相关时间范围内（秒）转化为乳酸和丙氨酸的非侵入性测量，有可能量化细胞的糖酵解状态。我们观察了P22肿瘤大鼠静脉注射超极化13c -丙酮酸后的代谢变化。超极化技术产生的强增强核磁共振信号允许使用1.5 T磁共振扫描仪在5 x 5 x 10 mm(3)成像体素中绘制丙酮酸盐、乳酸盐和丙氨酸。磁共振扫描（化学位移成像）在丙酮酸注射24秒后开始，持续时间为14秒。所有移植肿瘤的乳酸含量均明显高于正常组织。结果表明，局部Warburg效应的无创量化是可能的。
The "Warburg effect," an elevation in aerobic glycolysis, may be a fundamental property of cancer cells. For cancer diagnosis and treatment, it would be valuable if elevated glycolytic metabolism could be quantified in an image in animals and humans. The pyruvate molecule is at the metabolic crossroad for energy delivery inside the cell, and with a noninvasive measurement of the relative transformation of pyruvate into lactate and alanine within a biologically relevant time frame (seconds), it may be possible to quantify the glycolytic status of the cells. We have examined the metabolism after i.v. injection of hyperpolarized 13 C-pyruvate in rats with implanted P22 tumors. The strongly enhanced nuclear magnetic resonance signal generated by the hyperpolarization techniques allows mapping of pyruvate, lactate, and alanine in a 5 x 5 x 10 mm(3) imaging voxel using a 1.5 T magnetic resonance scanner. The magnetic resonance scanning (chemical shift imaging) was initiated 24 seconds after the pyruvate injection and had a duration of 14 seconds. All implanted tumors showed significantly higher lactate content than the normal tissue. The results indicate that noninvasive quantification of localized Warburg effect may be possible.