Chemical Engineering of Mesenchymal Stem Cells to Induce a Cell Rolling Response
Chemical Engineering of Mesenchymal Stem Cells to Induce a Cell Rolling Response
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DOI：
10.1021/bc800345q
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发表时间：
2008-11-01
影响因子：
4.7
通讯作者：
Karp, Jeffrey M.
中科院分区：
文献类型：
--
作者：
Sarkar, Debanjan;Vemula, Praveen Kumar;Karp, Jeffrey M.
在间充质干细胞（MSC）表面通过生物素-链亲和素桥接共价偶联的sialyl Lewis X （SLeX）在不改变细胞表型和多系分化潜能的情况下赋予白细胞样滚动特性。我们证明了SLeX在MSC表面的共轭是稳定的，通用的，并在p -选择素涂层的底物上诱导了强大的滚动响应。这些结果表明有可能提高任何细胞类型对特定组织的靶向效率。
Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin-streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX on the MSC surface is stable, versatile, and induces a robust rolling response on P-selectin coated substrates. These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue.