Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes
Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes
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DOI：
10.1007/s11626-014-9755-0
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发表时间：
2014-09-01
影响因子：
2.1
通讯作者：
Umezu, Mitsuo
中科院分区：
文献类型：
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作者：
Feng, Zhonggang;Takahashi, Rie;Umezu, Mitsuo
在这项研究中，我们研究了在常规单层培养的Wistar大鼠胚胎心室肌细胞中SRF-microRNA-1/microRNA-133 a-Hand 2通路的表达。培养的心肌细胞的形态学观察和三个重要的组成蛋白，MLC-2 v，N-cadherin，和connexin 43的mRNA表达水平，证明这些培养的细胞的不成熟，其特点是肌原纤维密度少，不成熟的肌节结构，和三个组成蛋白的mRNA表达显着低于新生儿心室样品。更重要的是，本研究的结果表明，SRF-microRNA-1/microRNA-133 a-Hand 2通路的改变导致培养的心肌细胞中Hand 2抑制的减弱。这些结果对于了解胚胎心肌细胞作为体外模型的细胞状态具有重要价值，并可能有助于评估工程化心脏组织和干细胞的心脏分化。
In this study, we investigated the expression of the pathway, SRF-microRNA-1/microRNA-133a-Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF-microRNA-1/microRNA-133a-Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.