MORN1 has a conserved role in asexual and sexual development across the Apicomplexa
MORN1 has a conserved role in asexual and sexual development across the Apicomplexa
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DOI：
10.1128/ec.00021-08
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发表时间：
2008-04-01
期刊：
影响因子：
--
通讯作者：
Gubbels, Marc-Jan
中科院分区：
文献类型：
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作者：
Ferguson, David J. P.;Sahoo, Nivedita;Gubbels, Marc-Jan
编码膜占据和识别连接蛋白 MORN1 的基因在整个顶端复合体中是保守的。在弓形虫中，MORN1 与纺锤体极、内膜复合体 (IMC) 的前环和后环相关。本研究探讨了 MORN1 在弓形虫和三种艾美耳球虫（在终宿主中）的球虫发育过程中以及恶性疟原虫红细胞分裂过程中的定位。在无性增殖过程中，MORN1 与艾美耳球虫和恶性疟原虫在弓形虫内多生殖和分裂过程中形成的多个子体的 IMC 后环相关。此外，恶性疟原虫MORN1蛋白的表达在分裂晚期达到顶峰。这些数据符合一个模型，该模型在无性发育的所有变体中 IMC 组装过程中 MORN1 的保守作用。一项重要的新观察结果是 MORN1 抗体与弓形虫和艾美耳球虫属某些性阶段的反应性。此处，MORN1 组织为环状结构，其中小配子从小配子细胞中萌芽，而在成熟的小配子中，它存在于鞭毛基体和线粒体附近。这些观察结果表明 MORN1 在整个顶复合体的无性和有性发育中都具有保守的作用。
The gene encoding the membrane occupation and recognition nexus protein MORN1 is conserved across the Apicomplexa. In Toxoplasma gondii, MORN1 is associated with the spindle poles, the anterior and posterior rings of the inner membrane complex (IMC). The present study examines the localization of MORN1 during the coccidian development of T. gondii and three Eimeria species (in the definitive host) and erythrocytic schizogony of Plasmodium falciparum. During asexual proliferation, MORN1 is associated with the posterior ring of the IMCs of the multiple daughters forming during T. gondii endopolygeny and schizogony in Eimeria and P. falciparum. Furthermore, the expression of P. falciparum MORN1 protein peaked in late schizogony. These data fit a model with a conserved role for MORN1 during IMC assembly in all variations of asexual development. An important new observation is the reactivity of MORN1 antibody with certain sexual stages in T. gondii and Eimeria species. Here MORN1 is organized as a ring-like structure where the microgametes bud from the microgametocyte while in mature microgametes it is present near the flagellar basal bodies and mitochondrion. These observations suggest a conserved role for MORN1 in both asexual and sexual development across the Apicomplexa.