A single pre-B cell can give rise to antigen-specific B cells that utilize distinct immunoglobulin gene rearrangements.
A single pre-B cell can give rise to antigen-specific B cells that utilize distinct immunoglobulin gene rearrangements.
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单个前 B 细胞可以产生利用不同免疫球蛋白基因重排的抗原特异性 B 细胞。
DOI：
10.1084/jem.172.3.815
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Caton,AJ
中科院分区：
文献类型：
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作者：
Caton,AJ
一组表达流感病毒血凝素（HA）相关特异性抗体的杂交瘤，其代表的B细胞是单个前B细胞的克隆后代，并且利用不同的L链基因重排。克隆关系是通过在生产性和非生产性等位基因上共享H链基因重排而建立的。在这些杂交瘤中，一组只重排了其tc个等位基因中的一个，将VK24基因连接到Jtc2基因片段上。另一组利用相同的Vtc24基因片段对jx5基因片段进行了生产性重排，并在非生产性K等位基因上进行了异常重排。因此，在同一个B细胞克隆的成员之间，不同的Ig基因重排的表达通常会发生，并且可以促进可用于识别外来抗原的免疫库的产生和多样化。讨论了杂交瘤表达的不同重排可能产生的机制。
A group of hybridomas that express antibodies with related specificities for the influenza virus hemagglutinin (HA), that represent B cells that were the clonal progeny of a single pre-B cell, and that utilized distinct L chain gene rearrangements have been characterized. The clonal relationship was established by the sharing of H chain gene rearrangements at both the productive and the nonproductive alleles. Among these hybridomas, one group had rearranged only one ofits tc alleles, having joined a VK24 gene to the Jtc2 gene segment. The other group utilized the same Vtc24 gene segment in productive rearrangement to theJx5 gene segment, and shared an aberrant rearrangement at the nonproductive K allele. Accordingly, the expression of distinct Ig gene rearrangements among members of the same B cell clone can normally occur, and can contribute to the generation and diversification of the immune repertoire that is available for the recognition of foreign antigens. Mechanisms by which the distinct rearrangements expressed by the hybridomas might have been generated are discussed.