IDENTIFICATION OF THE MAGNESIU, EUROPIUM AND LEAD BINDING-SITES IN ESCHERICHIA-COLI AND LUPINE TRANSFER RNAPHE BY SPECIFIC METAL ION-INDUCED CLEAVAGES
IDENTIFICATION OF THE MAGNESIU, EUROPIUM AND LEAD BINDING-SITES IN ESCHERICHIA-COLI AND LUPINE TRANSFER RNAPHE BY SPECIFIC METAL ION-INDUCED CLEAVAGES
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DOI：
10.1016/0014-5793(89)80148-6
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发表时间：
1989-01-30
期刊：
影响因子：
3.5
通讯作者：
KRZYZOSIAK, WJ
中科院分区：
文献类型：
--
作者：
MARCINIEC, T;CIESIOLKA, J;KRZYZOSIAK, WJ
Pb、Eu和Mg诱导的E.大肠杆菌和羽扇豆tRNAPhe已被表征，并与酵母tRNAPhe中发现的那些进行了比较。羽扇豆tRNAPhe的水解模式与酵母tRNAPhe的水解模式非常相似，但在E.大肠杆菌核糖核酸水解。后一种tRNA在D环中以低得多的产率被切割，并且有趣的是，在可变区中也检测到切割，其在真核tRNA中对水解具有高度抗性。讨论了E.根据具体的水解数据对大肠杆菌RNAPhe进行了讨论。
The Pb, Eu and Mg‐induced cleavages inE. coliand lupine tRNAPhehave been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhehydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of theE. colitRNAPhehydrolysis. The latter tRNA is cleaved with much lower yield in the D‐loop, and interestingly, cleavage is also detected in the variable region, that is highly resistant to hydrolysis in eukaryotic tRNAs. The possible location of tight Pb, Eu and Mg binding sites inE. colitRNAPheis discussed on the basis of the specific hydrolysis data.