Distribution of cuticular protein mRNAs in silk moth integument and imaginal discs.
Distribution of cuticular protein mRNAs in silk moth integument and imaginal discs.
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DOI：
10.1016/j.ibmb.2003.06.010
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发表时间：
2003-12
影响因子：
3.8
通讯作者：
S. Gu;J. H. Willis
中科院分区：
文献类型：
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作者：
S. Gu;J. H. Willis
采用RT-PCR和原位杂交技术检测了天蚕两种角质层蛋白mRNA的分布。对于幼虫和蛹角质层的主要区域，角质层类型与发现的主要角质层蛋白信息之间存在很强的对应关系。软角质层下的表皮细胞具有 HCCP12 的 mRNA，其中 RR-1 共有归因于软角质层，而与硬角质层相关的表皮细胞主要具有 HCCP66 的 mRNA，这是一种具有 RR-2 共有归因于硬角质层的蛋白质。这两种信息均在蛹前翅和后翅的所有区域中发现，区域特异性浓度差异远小于这些角质蛋白相对丰度的差异。 HCCP12 的 mRNA 存在于天蚕幼虫摄食的成虫盘中。 SilkBase (http://www.ab.a.u-tokyo.ac.jp/silkbase/) 上提供的来自家蚕的数据表明，取食幼虫的成虫盘含有丰富的 RR-1 角质层蛋白 mRNA，代表六种不同的基因产物。只有来自旋转幼虫的圆盘具有编码来自 10 个不同基因的 RR-2 蛋白的 mRNA。因此，鳞翅目翅成虫盘不能再被视为在幼虫角质层产生中不活跃。
The distributions of mRNAs for two cuticular proteins of Hyalophora cecropia were examined with RT-PCR and in situ hybridization. For major regions of larval and pupal cuticle, there was a strong correspondence between the type of cuticle and the predominant cuticular protein message found. Epidermal cells underlying soft cuticle had mRNA for HCCP12, with a RR-1 consensus attributed to soft cuticle, while the epidermal cells associated with hard cuticle had predominantly mRNA for HCCP66, a protein with the RR-2 consensus attributed to hard cuticle. Both messages were found in all areas of the pupal fore- and hind-wings, with modest area-specific difference in concentration being much less than differences in the relative abundance of these cuticular proteins. mRNA for HCCP12 was present in imaginal discs of feeding larvae of H cecropia. Data from Bombyx mori available at SilkBase (http://www.ab.a.u-tokyo.ac.jp/silkbase/) revealed that imaginal discs from feeding larvae had abundant mRNA for RR-1 cuticular proteins, representing six distinct gene products. Only discs from spinning larvae had mRNAs that coded for RR-2 proteins arising from 10 distinct genes. Thus, lepidopteran wing imaginal discs can no longer be regarded as inactive in larval cuticle production.