Genome and Ontogenetic-Based Transcriptomic Analyses of the Flesh Fly, Sarcophaga bullata
Genome and Ontogenetic-Based Transcriptomic Analyses of the Flesh Fly, Sarcophaga bullata
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DOI：
10.1534/g3.119.400148
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Ellen O. Martinson;J. Peyton;Yogeshwar D. Kelkar;Emily C. Jennings;J. Benoit;J. Werren;D. Denlinger
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文献类型：
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作者：
Ellen O. Martinson;J. Peyton;Yogeshwar D. Kelkar;Emily C. Jennings;J. Benoit;J. Werren;D. Denlinger
肉蝇是研究昆虫滞育、发育、胁迫耐受、神经生物学和寄主-寄生蜂相互作用等生理学的一个广泛使用的模型。在这个分类群中的蝇类与脊椎动物的幼虫感染有关，并以腐肉为食，这在法医学研究中是重要的方面。在这里，我们提出了S的基因组。bullata，沿着与发育和生殖为基础的RNA-Seq分析。我们预测了15，768个蛋白质编码基因，确定了与密切相关的果蝇的同源性，并根据我们的RNA-Seq分析建立了性别和发育特异性基因集。基因组序列、预测基因和测序数据集已存放在国家生物技术信息中心。我们的研究结果为基因组研究奠定了基础，这将扩大肉蝇作为模型系统的实用性。
The flesh fly, Sarcophaga bullata, is a widely-used model for examining the physiology of insect diapause, development, stress tolerance, neurobiology, and host-parasitoid interactions. Flies in this taxon are implicated in myiasis (larval infection of vertebrates) and feed on carrion, aspects that are important in forensic studies. Here we present the genome of S. bullata, along with developmental- and reproduction-based RNA-Seq analyses. We predict 15,768 protein coding genes, identify orthology in relation to closely related flies, and establish sex and developmental-specific gene sets based on our RNA-Seq analyses. Genomic sequences, predicted genes, and sequencing data sets have been deposited at the National Center for Biotechnology Information. Our results provide groundwork for genomic studies that will expand the flesh fly’s utility as a model system.