Effect of UV-photofunctionalization on oral bacterial attachment and biofilm formation to titanium implant material.
Effect of UV-photofunctionalization on oral bacterial attachment and biofilm formation to titanium implant material.
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DOI：
10.1016/j.biomaterials.2015.07.030
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发表时间：
2015-10
期刊：
影响因子：
14
通讯作者：
Lux R
中科院分区：
文献类型：
--
作者：
de Avila ED;Lima BP;Sekiya T;Torii Y;Ogawa T;Shi W;Lux R
细菌生物膜感染仍然是种植体失败的普遍原因。牙科种植体放置发生在口腔环境中，其中含有过多的生物膜形成细菌。由于其经粘膜放置，部分种植体结构暴露于口腔，并且没有有效的措施来防止细菌附着到种植体材料。在这里，我们证明了在使用（光功能化）之前立即对钛进行紫外线处理会影响人类多微生物口腔生物膜群落在唾液和血液成分存在下定殖的能力。紫外线处理的加工钛转化的表面从疏水性超亲水性。紫外线处理的表面表现出显着减少细菌附着以及随后的生物膜形成相比，未经处理的，即使整体细菌活力不受影响。在使用前储存在液体环境中的UV处理的钛上保持减少细菌定殖的功能。变性梯度凝胶电泳（DGGE）和DNA测序分析表明，虽然细菌群落配置文件之间出现不同的UV处理和未处理的钛在初始附着阶段，这种差异消失的生物膜形成的进展。我们的研究结果证实，钛的紫外光功能化具有很强的潜力，通过创建和保持抗菌表面来改善种植体放置的结果。
Bacterial biofilm infections remain prevalent reasons for implant failure. Dental implant placement occurs in the oral environment, which harbors a plethora of biofilm-forming bacteria. Due to its trans-mucosal placement, part of the implant structure is exposed to oral cavity and there is no effective measure to prevent bacterial attachment to implant materials. Here, we demonstrated that UV treatment of titanium immediately prior to use (photofunctionalization) affects the ability of human polymicrobial oral biofilm communities to colonize in the presence of salivary and blood components. UV-treatment of machined titanium transformed the surface from hydrophobic to superhydrophilic. UV-treated surfaces exhibited a significant reduction in bacterial attachment as well as subsequent biofilm formation compared to untreated ones, even though overall bacterial viability was not affected. The function of reducing bacterial colonization was maintained on UV-treated titanium that had been stored in a liquid environment before use. Denaturing gradient gel-electrophoresis (DGGE) and DNA sequencing analyses revealed that while bacterial community profiles appeared different between UV-treated and untreated titanium in the initial attachment phase, this difference vanished as biofilm formation progressed. Our findings confirm that UV-photofunctionalization of titanium has a strong potential to improve outcome of implant placement by creating and maintaining antimicrobial surfaces.