Comparing DNA damage-processing pathways by computer analysis of chromosome painting data.
Comparing DNA damage-processing pathways by computer analysis of chromosome painting data.
复制标题
通过计算机分析染色体绘制数据来比较 DNA 损伤处理途径。
DOI：
10.1089/cmb.2004.11.626
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Arsuaga,Javier
中科院分区：
文献类型：
--
作者：
Levy,Dan;Vazquez,Mariel;Cornforth,Michael;Loucas,Bradford;Sachs,RainerK;Arsuaga,Javier
Chromosome aberrations are large-scale illegitimate rearrangements of the genome. They are indicative of DNA damage and informative about damage processing pathways. Despite extensive investigations over many years, the mechanisms underlying aberration formation remain controversial. New experimental assays such as multiplex fluorescentin situhybridyzation (mFISH) allow combinatorial "painting" of chromosomes and are promising for elucidating aberration formation mechanisms. Recently observed mFISH aberration patterns are so complex that computer and graph-theoretical methods are needed for their full analysis. An important part of the analysis is decomposing a chromosome rearrangement process into "cycles." A cycle of ordern, characterized formally by the cyclic graph with2nvertices, indicates thatnchromatin breaks take part in a single irreducible reaction. We here describe algorithms for computing cycle structures from experimentally observed or computer-simulated mFISH aberration patterns. We show that analyzing cycles quantitatively can distinguish between different aberration formation mechanisms. In particular, we show that homology-based mechanisms do not generate the large number of complex aberrations, involving higher-order cycles, observed in irradiated human lymphocytes.
