Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
复制标题
鱼眼镜头中的稳定同位素作为营养和地理历史的潜在记录者
作者：
Amy A Wallace;D. Hollander;E. Peebles
我们评估了眼晶状体作为鱼类稳定同位素历史的潜在记录者，因为它们由代谢惰性光学蛋白组成，沉积在连续的同心圆（层）中，就像耳石环和树轮一样。我们对红鲷鱼、红石斑鱼、gag和white grunt的镜头进行了四种不同的测试。第一个试验是对多个个体进行低分辨率筛选（每个晶状体4-5个径向组的纹层，除白色grunt外的所有物种）。沿径向轴，所有个体均表现出显著的同位素变异性。红鲷鱼个体根据δ 15N分为两组，而红鲷鱼个体根据δ 13C分为两组。选择两个变异最大的gag进行高分辨率时间分析，使用来自第二眼透镜的单个层。来自高分辨率分析的一阶模式通常模仿低分辨率筛选成组层状的模式，但高分辨率图揭示了低分辨率筛选中不明显的早期生命细节。对于第三个测试，使用高分辨率方法比较左眼和右眼的变异。白须鱼左右眼的δ 13C和δ 15N的径向同位素模式几乎相同，这表明在单个鱼之间观察到的变化不是人为的。最后的测试评估层流内的变化；从同一层的不同部位分析多个样品。用这种方法分析了2种3个个体的7个纹层；发现层间的变化比层内的变化要大得多。然而，标称的层流内变化与左右透镜之间的标称差异相当，表明层流内变化确定了测量精度。眼晶状体同位素似乎对重建单个鱼类的同位素历史很有用；这些历史可以与空间衍生的等值线图进行比较，以重建场地保真度，运动和营养位置的个体历史。
We evaluated eye lenses as potential recorders of stable isotope histories in fish because they consist of metabolically inert optical proteins that are deposited in successive, concentric circles (laminae) much like otolith circuli and tree rings. We conducted four different tests on lenses from red snapper, red grouper, gag, and white grunt. The first test was a low-resolution screening of multiple individuals (4–5 radial groups of laminae per lens, all species except white grunt). Along the radial axis, all individuals exhibited substantial isotopic variability. Red snapper individuals separated into two groups based on δ 15N and gag separated into two groups based on δ 13C. Two gag with the greatest variation were chosen for high-resolution temporal analysis using individual laminae from their second eye lenses. The first-order patterns from the high-resolution analysis generally mimicked patterns from the low-resolution screening of grouped laminae, yet the high-resolution plots revealed early-life details that were not apparent in the low-resolution screenings. For the third test, left- versus right-eye variation was compared using high-resolution methods. White grunt left- and right-eye radial isotopic patterns were almost identical for both δ 13C and δ 15N, suggesting the variations observed among individual fish were not artifacts. The final test evaluated intra-laminar variation; multiple samples were analyzed from different parts of the same lamina. Seven laminae from three individuals of two species were analyzed in this manner; variations among laminae were found to be much higher than variations within laminae. However, nominal intra-laminar variations were comparable to nominal differences between left and right lenses, suggesting intra-laminar variation established measurement precision. Eye lens isotopes appear to be useful for reconstructing the isotopic histories of individual fish; these histories can be compared with spatially-derived isoscapes to reconstruct individual histories for site fidelity, movement and trophic position.
DOI：
10.1016/j.bbagen.2009.05.008
发表时间：
2009-10
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Sharma KK;Santhoshkumar P
通讯作者：
Santhoshkumar P