Identification of protein-coding and non-coding RNA expression profiles in CD34+ and in stromal cells in refractory anemia with ringed sideroblasts
Identification of protein-coding and non-coding RNA expression profiles in CD34+ and in stromal cells in refractory anemia with ringed sideroblasts
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DOI：
10.1186/1755-8794-3-30
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发表时间：
2010-07-15
影响因子：
2.7
通讯作者：
Olalla-Saad, Sara T.
中科院分区：
文献类型：
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作者：
Baratti, Mariana O.;Moreira, Yuri B.;Olalla-Saad, Sara T.
工作背景：骨髓增生异常综合征（MDS）是一组克隆性血液病，其特征是造血功能低下，骨髓细胞发育异常，导致外周血细胞减少。微阵列技术已经允许在人类基因组中的转录活性的精细的高通量映射。从基因的内含子区域转录的非编码RNA（ncRNA）参与许多与基因表达的转录后控制相关的过程，并且参与外显子跳跃和内含子保留的调节。MDS患者的祖细胞和基质细胞中ncRNA的表征对于理解这种疾病中的基因表达调控可能是战略性的。在本研究中，应用44 k内含子-内含子组合技术，对4例伴有环形铁粒幼细胞（RARS）亚群的难治性贫血MDS患者的CD 34（+）细胞和3例MDS-RARS患者的基质细胞基因表达谱进行了比较。外显子oligoarrays，包括蛋白质编码基因外显子的探针，以及从有义链或反义链的内含子区域转录的非编码RNA的探针。结果：MDS-RARS患者的CD 34（+）细胞中，有216个基因表达差异显著（q值
Background: Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders characterized by ineffective hematopoiesis with morphological evidence of marrow cell dysplasia resulting in peripheral blood cytopenia. Microarray technology has permitted a refined high-throughput mapping of the transcriptional activity in the human genome. Non-coding RNAs (ncRNAs) transcribed from intronic regions of genes are involved in a number of processes related to post-transcriptional control of gene expression, and in the regulation of exon-skipping and intron retention. Characterization of ncRNAs in progenitor cells and stromal cells of MDS patients could be strategic for understanding gene expression regulation in this disease.Methods: In this study, gene expression profiles of CD34(+) cells of 4 patients with MDS of refractory anemia with ringed sideroblasts (RARS) subgroup and stromal cells of 3 patients with MDS-RARS were compared with healthy individuals using 44 k combined intron-exon oligoarrays, which included probes for exons of protein-coding genes, and for non-coding RNAs transcribed from intronic regions in either the sense or antisense strands. Real-time RT-PCR was performed to confirm the expression levels of selected transcripts.Results: In CD34(+) cells of MDS-RARS patients, 216 genes were significantly differentially expressed (q-value