Peripheral blood levels of immunoreactive inhibin during pseudopregnancy, pregnancy and lactation in the rat.
Peripheral blood levels of immunoreactive inhibin during pseudopregnancy, pregnancy and lactation in the rat.
复制标题
大鼠假孕、妊娠和哺乳期间免疫反应性抑制素的外周血水平。
作者：
K. Taya;H. Komura;G. Watanabe;S. Sasamoto
用放射免疫法测定了假孕、孕鼠和哺乳期大鼠外周血中抑制素的浓度，并与卵泡发育状况、卵巢黄体功能和胎盘功能进行了相关分析。在假孕大鼠中，从假孕第2天到第12天，抑制素的血浆浓度保持在相对较高的水平，并且在整个假孕期间没有明显的变化。在怀孕大鼠中，血浆抑制素浓度在第6天到第14天之间显著下降，随后在第20天和第21天突然增加。在哺乳大鼠中，哺育八只幼崽的母鼠的血浆抑制素浓度始终低于哺育两只幼崽的母鼠。哺乳早期血浆抑制素浓度较低，哺乳后期显着升高。哺乳期抑制素的变化与血浆黄体生成素浓度呈正相关，与雌二醇17β、睾酮、孕酮浓度无相关性。另一方面，在假孕、妊娠和哺乳期间，血浆FSH浓度均在发情水平范围内。这些结果表明，大鼠黄体期外周血浆抑制素浓度随健康有腔卵泡数目的不同而不同，与黄体功能无明显正相关。结果还表明，抑制素是大鼠卵泡发育的比雌二醇-17β更直接的指标。
Concentrations of inhibin in peripheral blood were determined by radioimmunoassay in pseudopregnant, pregnant and lactating rats and correlated with the status of follicular development, luteal function in the ovaries and placental function. In pseudopregnant rats, plasma concentrations of inhibin remained relatively high from days 2 to 12 of pseudopregnancy and no significant changes occurred throughout pseudopregnancy. In pregnant rats, a marked decline in plasma concentrations of inhibin occurred between days 6 and 14 followed by an abrupt increase on days 20 and 21. In lactating rats, plasma concentrations of inhibin were consistently lower in dams nursing eight pups than in dams nursing two pups. Plasma concentrations of inhibin remained low during the early stages of lactation followed by a significant increase during the late stages in dams nursing eight pups. These changes in inhibin during the period of lactation correlated with those of plasma concentrations of LH, but not with those of oestradiol-17 beta, testosterone and progesterone. Throughout pseudopregnancy, pregnancy and lactation, on the other hand, plasma concentrations of FSH were within the range of dioestrous levels. These findings indicate that concentrations of inhibin in peripheral plasma vary with the number of healthy antral follicles and no positive correlation was observed with luteal function during the luteal phase in rats. The results also suggest that inhibin is a more direct index of follicular development than oestradiol-17 beta in rats.