Structural organisation of phycobilisomes from Synechocystis sp strain PCC6803 and their interaction with the membrane
Structural organisation of phycobilisomes from Synechocystis sp strain PCC6803 and their interaction with the membrane
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DOI：
10.1016/j.bbabio.2009.01.009
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发表时间：
2009-04-01
影响因子：
4.3
通讯作者：
Boekema, Egbert J.
中科院分区：
文献类型：
--
作者：
Arteni, Ana A.;Ajlani, Ghada;Boekema, Egbert J.
在蓝藻中，光合作用所需光能的收集主要是由藻胆体（一种巨大的、多亚基的色素蛋白复合体）完成的。该复合物由异二聚体藻胆蛋白组成，所述异二聚体藻胆蛋白借助于接头多肽组装，使得光吸收和向光系统II的能量转移得到优化。在这项工作中，我们研究了，使用单粒子电子显微镜，藻胆体结构的突变体缺乏两个或所有三个的藻蓝蛋白六聚体。所呈现的图像比先前发表的图像提供了更多的细节，并且在最好的二维投影图中，分辨率达到了13埃。以及给一个更好的整体图片的组装藻胆体，这些结果揭示了新的细节内的核心别藻蓝蛋白三聚体的协会。深入了解这个核心的膜表面，有效的能量转移到光系统II的附件。有和没有重组铁氧还蛋白：NADP氧化还原酶的藻胆体的投影图的比较表明，该酶在杆-芯界面的复杂的位置。(C)2009 Elsevier B. V.保留所有权利。
In cyanobacteria, the harvesting of light energy for photosynthesis is mainly carried out by the phycobilisome a giant, multi-subunit pigment-protein complex. This complex is composed of heterodimeric phycobiliproteins that are assembled with the aid of linker polypeptides such that light absorption and energy transfer to photosystem II are optimised. In this work we have studied, using single particle electron microscopy, the phycobilisome structure in mutants lacking either two or all three of the phycocyanin hexamers. The images presented give much greater detail than those previously published, and in the best two-dimensional projection maps a resolution of 13 angstrom was achieved. As well as giving a better overall picture of the assembly of phycobilisomes, these results reveal new details of the association of allophycocyanin trimers within the core. Insights are gained into the attachment of this core to the membrane surface, essential for efficient energy transfer to photosystem II. Comparison of projection maps of phycobilisomes with and without reconstituted ferredoxin:NADP oxidoreductase suggests a location for this enzyme within the complex at the rod-core interface. (C) 2009 Elsevier B.V. All rights reserved.