Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.
Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.
复制标题
分布式干细胞中死亡染色体上的 H3K27 和 H3K4 三甲基化减少。
DOI：
10.1038/cddis.2014.522
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发表时间：
2014
影响因子：
9
通讯作者：
Sherley,JL
中科院分区：
文献类型：
--
作者：
Huh,YH;Sherley,JL
在不对称自我更新分布式干细胞 (DSC) 有丝分裂期间共分离的永生 DNA 链的作用尚不清楚。此前，由于难以鉴定 DSC 和永生 DNA 链，因此无法研究永生 DNA 链功能和导致其非随机共分离的分子机制。在这里，我们报告了两项技术创新的使用，选择性 DSC 扩展和 H2A 的建立。 Z 染色体不对称性作为“永生染色体”的特定标记，研究培养的小鼠毛囊 DSC 中永生染色体和相反“永生染色体”的分子特性。尽管永生染色体上各自抑制性和激活性 H3K27me3 和 H3K4me3 表观遗传标记的检测与随机分离的染色体相似，但在注定用于谱系定型姐妹细胞的永生染色体上，两者的检测均较低。这种非随机共分离的全局表观基因组特征可能揭示了一种维持表观基因组范围“平衡”转录状态的机制，该状态保留了 DSC 身份，同时激活姐妹染色体进行分化。
The role of immortal DNA strands that co-segregate during mitosis of asymmetrically self-renewing distributed stem cells (DSCs) is unknown. Previously, investigation of immortal DNA strand function and molecular mechanisms responsible for their nonrandom co-segregation was precluded by difficulty in identifying DSCs and immortal DNA strands. Here, we report the use of two technological innovations, selective DSC expansion and establishment of H2A. Z chromosomal asymmetry as a specific marker of ‘immortal chromosomes,’to investigate molecular properties of immortal chromosomes and opposing ‘mortal chromosomes’ in cultured mouse hair follicle DSCs. Although detection of the respective suppressive and activating H3K27me3 and H3K4me3 epigenetic marks on immortal chromosomes was similar to randomly segregated chromosomes, detection of both was lower on mortal chromosomes destined for lineage-committed sister cells. This global epigenomic feature of nonrandom co-segregation may reveal a mechanism that maintains an epigenome-wide ‘poised’transcription state, which preserves DSC identity, while simultaneously activating sister chromosomes for differentiation.