Cytotoxic antibodies to cloned rat islet cells in serum of patients with diabetes mellitus.
Cytotoxic antibodies to cloned rat islet cells in serum of patients with diabetes mellitus.
复制标题
糖尿病患者血清中克隆大鼠胰岛细胞的细胞毒性抗体。
DOI：
10.1172/jci110048
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Scearce,RM
中科院分区：
文献类型：
--
作者：
Eisenbarth,GS;Morris,MA;Scearce,RM
我们使用单层克隆大鼠胰岛细胞（克隆 RINm 5F 和 RINm 14B）进行 51Cr 细胞毒性测定，在 24 名胰岛素依赖性糖尿病患者中的 8 名患者的血清中发现了补体依赖性细胞毒性抗体。相比之下，在 34 名对照者或 5 名非糖尿病多腺功能衰竭患者的血清中发现了 51Cr 释放大于 24% 的补体依赖性细胞毒性，并且在 12 名非胰岛素依赖型糖尿病患者的血清中仅存在 1 份血清。抗体的患病率似乎随着胰岛素依赖型糖尿病的持续时间而降低，在一名研究的患者中，细胞毒性抗体在诊断糖尿病时就存在。细胞毒性与胰岛素合成无关，产生胰岛素的克隆 RINm 5F 和产生生长抑素的克隆 RINm 14B 的血清细胞毒性的线性相关性证明了这一点。本研究鉴定了糖尿病患者血清中的非物种特异性细胞毒性抗体，所使用的检测方法应有助于研究糖尿病发病机制中的体液免疫。
We have found complement-dependent cytotoxic antibodies in the serum of 8 of 24 patients with insulin-dependent diabetes mellitus using a 51Cr cytotoxicity assay with monolayers of cloned rat islet cells (clones RINm 5F and RINm 14B). In contrast, complement-dependent cytotoxicity with 51Cr release greater than 24% was found with sera from 34 controls or from 5 patients with polyglandular failure without diabetes, and was present in only 1 serum our of 12 from patients with insulin-independent diabetes mellitus. The prevalence of antibodies appears to decrease with duration of insulin-dependent diabetes, and in one patient studied, cytotoxic antibodies were present at the time of diagnosis of diabetes. Cytotoxicity is independent of insulin synthesis, as evidenced by the linear correlation of cytotoxicity of sera for the insulin-producing clone RINm 5F and the somatostatin-producing clone RINm 14B. The present study identifies nonspecies-specific cytotoxic antibodies in the serum of patients with diabetes mellitus, and the assay used should facilitate studies of humoral immunity in the pathogenesis of diabetes mellitus.Images