A cis-element responsible for cGMP in the promoter of the soybean chalcone synthase gene.
A cis-element responsible for cGMP in the promoter of the soybean chalcone synthase gene.
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DOI：
10.1016/j.plaphy.2013.10.034
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
H. Abu Zahra;Satoru Kuwamoto;T. Uno;K. Kanamaru;H. Yamagata
中科院分区：
文献类型：
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作者：
H. Abu Zahra;Satoru Kuwamoto;T. Uno;K. Kanamaru;H. Yamagata
环核苷酸cGMP和cAMP已被报道在植物过程和反应的调节中发挥关键作用。我们已经报道了一些编码类黄酮类生物合成酶的基因，包括大豆查尔酮合成酶(CHS)，被cGMP诱导，而不是cAMP。大豆基因组包含9个CHS基因拷贝(GmCHS1-9)。我们研究了几个GmCHS基因对cGMP、cAMP、NO和白光的响应。定量RT-PCR分析表明，加入cGMP后，GmCHS7和GmCHS8的转录水平分别增加了3.6倍和3.8倍，而GmCHS2的转录水平保持不变。虽然cAMP对这三个基因的转录水平没有影响，但NO对这三个基因都有激活作用。白光瞬间激活这三个基因，GmCHS2、GmCHS7和GmCHS8的转录水平在3h后增加3倍，9h后降至基础水平，GmCHS8启动子包含几个重要的顺式元件，包括形成单位I类序列的G-box和H-box，以及GmMYB176转录因子调控GmCHS8表达的靶标MYB结合序列。瞬时基因表达分析表明，cGMP激活了单位I类序列，但不激活了MYB结合序列。G-box和H-box的结合对于cGMP的应答是必要的。综上所述，这些结果表明，GmCHS7和GmCHS8启动子中的类单位I序列是这两个基因中cGMP应答元件，NO通过除类单位I序列以外的元件发挥作用。
The cyclic nucleotides cGMP and cAMP have been reported to play key roles in the regulation of plant processes and responses. We have previously reported that several genes encoding flavonoid biosynthetic enzymes, including chalcone synthase (CHS) in soybean (Glycine maxL.), were induced by cGMP but not cAMP. The soybean genome contains nine CHS gene copies (GmCHS1–9). We investigated the responsiveness of several GmCHS genes to cGMP, cAMP, NO, and white light. Quantitative RT-PCR analysis showed that the transcript levels ofGmCHS7andGmCHS8were increased by 3.6- and 3.8-fold, respectively, with cGMP whereas the transcript levels ofGmCHS2remained constant. Although cAMP had no effect on the transcript levels of the three genes, NO had an activation effect on all three. White light activated the three genes in a transient manner, withGmCHS2,GmCHS7, andGmCHS8transcript levels increasing 3-fold after 3 h and decreasing to basal levels after 9 h. TheGmCHS8promoter contains several importantcis-elements, including the G-box and H-box forming the Unit-I-like sequence and the MYB binding sequence, a target of the GmMYB176 transcription factor regulating the expression ofGmCHS8. A transient gene expression assay revealed the activation of the Unit-I-like sequence, but not of the MYB binding sequence, by cGMP. The combination of G-box and H-box was necessary for cGMP responsiveness. Taken together, these results suggest that the Unit-I-like sequence in the promoters ofGmCHS7andGmCHS8is a cGMP responsivecis-element in these genes and that NO exerts its effect viacis-elements other than the Unit-I-like sequence.