Tumor osteolysis in osteopetrotic mice
Tumor osteolysis in osteopetrotic mice
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DOI：
10.1002/jor.1100130613
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发表时间：
1995-11-01
影响因子：
2.8
通讯作者：
Ramnaraine, MLR
中科院分区：
文献类型：
--
作者：
Clohisy, DR;Ogilvie, CM;Ramnaraine, MLR
肿瘤骨质溶解部位骨局部破坏所涉及的细胞和生化机制的细节尚不清楚。研究表明，来自肉瘤 (2472) 细胞系的肿瘤通过刺激破骨细胞的形成和激活，诱导小鼠局部骨质溶解。本报告研究了2472个肿瘤对破骨细胞缺陷动物（op/op）骨骼的影响，op/op股骨接种2472细胞后，肿瘤生长并发生局灶性骨溶解。假注射股骨中每个组织学切片的破骨细胞数 (19 +/- 5) 比荷瘤股骨中的破骨细胞数 (412 +/- 129) 多 (p < 0.05)。破骨细胞的大小也从假注射肢体中的 304 +/- 81 μ m(2) 增加到荷瘤肢体中的 407 +/- 62 μ m(2) (p < 0.001)。来自 2472 个 op/op 肿瘤外植体的条件培养基含有巨噬细胞集落刺激因子。 op/op 小鼠中破骨细胞的缺乏是由于缺乏该因子造成的；因此，这些数据表明源自2472肿瘤的巨噬细胞集落刺激因子可能负责在肿瘤部位引导破骨细胞介导的骨溶解。
Details of the cellular and biochemical mechanisms involved in focal destruction of bone at sites of tumor osteolysis are unknown. It has been shown that tumors from sarcoma (2472) cell lines induce focal osteolysis in mice by stimulating formation and activation of osteoclasts. In this report, the influence of 2472 tumors on the skeletons of osteoclast-deficient animals (op/op) was studied, After op/op femora had been inoculated with 2472 cells, tumors developed and focal osteolysis occurred. There were more osteoclasts per histologic section in sham-injected femora (19 +/- 5) than in tumor-bearing femora (412 +/- 129) (p < 0.05). The size of the osteoclasts also increased from 304 +/- 81 mu m(2) in sham-injected limbs to 407 +/- 62 mu m(2) in tumor-bearing limbs (p < 0.001). Conditioned media from 2472 op/op tumor explants contained macrophage colony-stimulating factor. A deficiency of osteoclasts in op/op mice is the result of the absence of this factor; therefore, these data introduce the possibility that macrophage colony-stimulating factor derived from 2472 tumor may be responsible for directing osteoclast-mediated osteolysis at sites of the tumor.