Internalization and Phytotoxic Effects of CuO Nanoparticles in Arabidopsis thaliana as Revealed by Fatty Acid Profiles.
Internalization and Phytotoxic Effects of CuO Nanoparticles in Arabidopsis thaliana as Revealed by Fatty Acid Profiles.
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DOI：
10.1021/acs.est.6b02613
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发表时间：
2016-09
影响因子：
11.4
通讯作者：
Jin Yuan;A. He;Shi-Qi Huang;Jing Hua;G. Sheng
中科院分区：
文献类型：
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作者：
Jin Yuan;A. He;Shi-Qi Huang;Jing Hua;G. Sheng
从细胞水平研究了纳米氧化铜（nCuO）在植物体内的内化和植物毒性效应。拟南芥水培挑战nCuO（100 mg/L），相比Cu 2+离子（1.2 mg/L），占nCuO的溶解96小时和28天，监测铜积累在植物以及植物细胞膜的脂肪酸（FA）配置文件。在相同的生长条件下，nCuO暴露导致更多的Cu积累比Cu 2+暴露。多种显微镜技术证实了内在化和螯合的nCuO在根细胞液泡，在那里发生转化的Cu（II）的Cu（I）Cl。短期和长期曝光（96小时与28天），以nCuO和Cu 2+提高FA饱和度在植物细胞通过氧化应激，验证了超氧自由基的原位检测，转换主要从C18：3，C16：3，和C18：2到C16：0。只有长时间暴露于nCuO才能显著提高根细胞中FA的饱和度。这些结果表明，nCuO对植物的急性效应主要来自于nCuO溶解释放的Cu 2+离子的胁迫，而对根系的慢性效应则主要来自于nCuO颗粒的胁迫。这项工作中的发现是新颖的，可能对更好地理解纳米颗粒诱导的植物毒性和生态系统中的潜在风险具有重要意义。
Internalization and phytotoxic effects of CuO nanoparticles (nCuO) in plants were studied at the cellular level. Arabidopsis thaliana was hydroponically challenged by nCuO (100 mg/L), as compared to Cu2+ ions (1.2 mg/L), to account for nCuO dissolution for 96 h and 28 days to monitor Cu accumulation in the plant as well as the fatty acid (FA) profiles of the plant cell membrane. Under the same growing conditions, the nCuO exposure resulted in more Cu accumulation than did the Cu2+ exposure. Multiple microscopic techniques confirmed the internalization and sequestration of nCuO in root cell vacuoles, where transformation of Cu(II) to Cu(I)Cl occurred. Short and long exposures (96 h versus 28 days) to both nCuO and Cu2+ elevated FA saturation degrees in plant cells through oxidative stress, as verified by in situ detection of superoxide radicals, with conversions mostly from C18:3, C16:3, and C18:2 to C16:0. Only the long exposure to nCuO significantly brought about an additional elevation of FA saturation degree in root cells. These results demonstrated that the acute effects of plant exposure to nCuO were mainly produced from the stress of Cu2+ ions released from nCuO dissolution, while the chronic effects in roots were significantly developed by the nCuO particle stress. The findings in this work are novel and may offer significant implications in better understanding nanoparticle-induced phytotoxicity and potential risks in ecosystems.