Analysis of genetic markers of N. Gonorrhoeae resistance to β-lactam antibiotics
Analysis of genetic markers of N. Gonorrhoeae resistance to β-lactam antibiotics
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淋球菌对β-内酰胺类抗生素耐药的遗传标记分析
DOI：
10.1007/s10517-006-0234-9
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发表时间：
2006
影响因子：
0.7
通讯作者：
A. Kubanova
中科院分区：
文献类型：
--
作者：
M. Malakhova;V. A. Vereshchagin;E. N. Il’ina;V. Govorun;M. Zubkov;T. Priputnevich;V. Kisina;A. Kubanova
建立了一种检测N.淋病对青霉素产生了耐药性。通过微测序反应检测penA和ponA基因的突变，随后通过MALDI-TOF质谱检测反应产物。该方法在31株临床菌株上进行了测试。淋病，青霉素的最低抑制浓度为0.03 - 8 µg/ml或更高。在中度耐药菌株中显示了penA和ponA基因的突变（最小抑制浓度高达0.5 μg/ml），在耐药菌株中显示了penA、ponA和penB基因的突变（最小抑制浓度大于1.0 μg/ml）。4株高耐药菌株（最低抑菌浓度4-8 μg/ml及以上）检出β-内酰胺酶基因。检测了所研究位点中微生物耐药性与相应突变存在之间的相关性。
A complex method for detection of genetic markers ofN. gonorrhoeaeresistance to penicillin was developed. Mutations inpenAandponAgenes were detected by minisequencing reaction with subsequent detection of reaction products by MALDI-TOF mass spectrometry. This approach was tested on 31 clinical strains ofN. gonorrhoeaewith minimum inhibitory concentration of penicillin from 0.03 to 8 µg/ml and higher. Mutations inpenAandponAgenes in moderately resistant strains were shown (minimum inhibitory concentration up to 0.5 µg/ml) and mutations inpenA, ponA, andpenBgenes in resistant strains (minimum inhibitory concentration more than 1.0 µg/ml). β-Lactamase genes were detected in 4 strains with high resistance (minimum inhibitory concentration 4–8 and more µg/ml). Correlation between microbiological resistance and presence of respective mutations in the studied locuses was detected.
影响因子：
9.4
作者：
BOOM, R;SOL, CJA;VANDERNOORDAA, J
通讯作者：
VANDERNOORDAA, J