THE INVECTED GENE OF DROSOPHILA - SEQUENCE-ANALYSIS AND EXPRESSION STUDIES REVEAL A CLOSE KINSHIP TO THE ENGRAILED GENE
THE INVECTED GENE OF DROSOPHILA - SEQUENCE-ANALYSIS AND EXPRESSION STUDIES REVEAL A CLOSE KINSHIP TO THE ENGRAILED GENE
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DOI：
10.1101/gad.1.1.19
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发表时间：
1987-03-01
影响因子：
10.5
通讯作者：
KORNBERG, T
中科院分区：
文献类型：
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作者：
COLEMAN, KG;POOLE, SJ;KORNBERG, T
侵染基因和嵌合基因并列在果蝇基因组中，并且在序列和表达模式上密切相关。通过获得全长cDNA序列，通过对S1核酸酶敏感性和引物延伸的研究，确定了最丰富的转录本的结构，确定了目的基因的部分序列，并通过Northern分析和原位定位鉴定了目的基因的发育情况。被感染的基因和嵌合基因一样，在胚胎和后发育间隔的幼虫细胞中表达，在胚胎后肠、盲肠和神经系统中表达。与增强型基因一样，被感染的基因可以编码一个约60kD的蛋白质，在其羧基末端附近含有一个同源异位盒；实际上，两种蛋白质的羧基末端区域的117个氨基酸序列几乎相同。被导入的基因的发育作用尚不清楚。
The invected and engrailed genes are juxtaposed in the Drosophila genome and are closely related in sequence and pattern of expression. The structure of the most abundant invected transcript was defined by obtaining the full-length cDNA sequence and by S1 nuclease sensitivity and primer extension studies; a partial sequence of the invected gene was determined; and the development profile of invected expression was characterized by Northern analysis and by in situ localization. The invected gene, like the engrailed gene, is expressed in the embryonic and larval cells of the posterior development compartments and in the embryonic hindgut, clypeolabrum, and nervous system. Like the engrailed gene, the invected gene can encode a protein of approximately 60 kD that contains a homeo box near its carboxyl terminus; indeed, a sequence of 117 amino acids in the carboxy-terminal region of both proteins is almost identical. The developmental role of the invected gene is not known.