2-Aminopurine-Modified Abasic-Site-Containing Duplex DNA for Highly Selective Detection of Theophylline
2-Aminopurine-Modified Abasic-Site-Containing Duplex DNA for Highly Selective Detection of Theophylline
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DOI：
10.1021/ja8095625
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发表时间：
2009-02-25
影响因子：
15
通讯作者：
Teramae, Norio
中科院分区：
文献类型：
--
作者：
Li, Minjie;Sato, Yusuke;Teramae, Norio
2-氨基嘌呤修饰的含碱基双链[DNA 5'-TCTGC GTCCT (PX)]在barT TAACG下CACAC-3'/3'-AGACG CAGGA T (C)在barA下ATTGC GTGTG-5'；(P) bar = 2-氨基嘌呤，(X) bar =碱基（Spacer-C3）， (C) bar =受体碱基]能够选择性结合支气管扩张剂茶碱，解离常数为10 μ M(5℃，pH 7.0, I = 0.11 M)，适用于监测血清茶碱浓度。
2-Aminopurine-modified abasic-site-containing duplex [DNA 5'-TCTGC GTCCT (PX) under barT TAACG CACAC-3'/3'-AGACG CAGGA T (C) under barA ATTGC GTGTG-5'; (P) under bar = 2-aminopurine, (X) under bar = abasic site (Spacer-C3), (C) under bar = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 mu M (5 degrees C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.