ToxR regulates the production of lipoproteins and the expression of serum resistance in Vibrio cholerae.
ToxR regulates the production of lipoproteins and the expression of serum resistance in Vibrio cholerae.
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DOI：
10.1073/pnas.88.5.1641
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发表时间：
1991-03
影响因子：
11.1
通讯作者：
C. Parsot;E. Taxman;John J. MEKALANOSt
中科院分区：
文献类型：
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作者：
C. Parsot;E. Taxman;John J. MEKALANOSt
采用DNA序列分析和TnphoA基因融合编码的杂交蛋白[3H]棕榈酸盐标记相结合的方法，鉴定了霍乱弧菌3种脂蛋白的编码基因。TagA、AcfD和TcpC这三种脂蛋白的表达受霍乱毒素转录激活因子ToxR的控制。其他细菌脂蛋白参与赋予对补体杀菌作用的抗性，促使我们在霍乱弧菌中研究这种可能性。值得注意的是，参与毒素共调控菌毛生物发生的toxR和tcp基因（包括tcpC）的突变，使霍乱弧菌对抗体和补体的杀弧菌活性的敏感性提高了约10(4)-10(6)倍。由于霍乱弧菌是一种非侵入性生物，而toxR和tcp突变体在动物和人类的肠道定植中存在高度缺陷，因此这些结果提出了一种可能性，即对肠道相关的“补体样”杀菌活性的耐药性可能是霍乱弧菌和其他肠道细菌物种的主要毒力决定因素。
The genes encoding three lipoproteins of Vibrio cholerae were identified by a combination of DNA sequence analysis and [3H]palmitate labeling of hybrid proteins encoded by TnphoA gene fusions. The expression of these three lipoproteins, TagA, AcfD, and TcpC, was controlled by ToxR, the cholera toxin transcriptional activator. The involvement of other bacterial lipoproteins in conferring resistance to the bactericidal effects of complement prompted us to examine this possibility in V. cholerae. Remarkably, mutations in toxR and tcp genes (including tcpC), involved in the biogenesis of the toxin coregulated pili, rendered V. cholerae about 10(4)-10(6) times more sensitive to the vibriocidal activity of antibody and complement. Since V. cholerae is a noninvasive organism and toxR and tcp mutants are highly defective in intestinal colonization in animals and humans, these results raise the possibility that resistance to a gut-associated, "complement-like" bactericidal activity may be a major virulence determinant of V. cholerae and other enterobacterial species.