Genetic and epigenetic X-chromosome variations in a parthenogenetic human embryonic stem cell line
Genetic and epigenetic X-chromosome variations in a parthenogenetic human embryonic stem cell line
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孤雌生殖人胚胎干细胞系中的遗传和表观遗传 X 染色体变异
DOI：
10.1007/s10815-010-9517-1
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发表时间：
2011-04-01
影响因子：
3.1
通讯作者：
Sun, Xiaofang
中科院分区：
文献类型：
--
作者：
Liu, Weiqiang;Yin, Yifei;Sun, Xiaofang
目的探讨孤雌生殖人胚胎干细胞（phESCs）的遗传学和表观遗传学状态，方法对本实验室分离的一株phESCs（FY-phES-018）进行细胞遗传学、X染色体失活（XCI）和基因表达谱分析。这些细胞具有46，XX核型，并且在第20代之前未观察到失活X染色体。体外长期培养后，部分细胞丢失一个X，且仅有一个X的细胞比例逐渐增加。在第35代，几乎所有的细胞显示45，XO核型。有趣的是，在第45代，观察到X染色体的恢复，并且XCI变得可检测; 46，XX与45，XO的嵌合体比率为67：33。在第60代后，大多数细胞再次显示46，XX核型，嵌合比为97：3。一些异常的基因组印迹也观察到在这些cells. ConclusionsphESCs系FY-phES-018是遗传和表观遗传不稳定的，因此，需要进一步的研究，然后再使用这些细胞。
PurposeTo assess the genetic and epigenetic status of parthenogenetic human embryonic stem cells (phESCs).MethodsCytogenetics, X chromosome inactivation (XCI) and gene expression patterns were analyzed in one phESC line (FY-phES-018) that was derived from our laboratory.ResultsFY-phES-018 cells displayed the classical characteristics of normal hESCs. These cells had a 46, XX karyotype, and no inactive X chromosomes were observed before passage 20. After being cultured long term in vitro, some cells lost one X, and the proportion of cells with only one X gradually increased. At passage 35, almost all the cells displayed a 45, XO karyotype. Interestingly, at passage 45, the recovery of the X-chromosome was observed, and XCI became detectable; the mosaic ratio of 46, XX to 45, XO was 67:33. After passage 60, most cells displayed the 46, XX karyotype again with a mosaic ratio of 97:3. Some aberrant genomic imprinting was also observed in these cells.ConclusionsThe phESCs line FY-phES-018 is both genetically and epigenetically unstable; therefore, further research is needed before using these cells.