COMPARISON OF CHLOROPLAST DNAS BY SPECIFIC FRAGMENTATION WITH ECORI ENDONUCLEASE
COMPARISON OF CHLOROPLAST DNAS BY SPECIFIC FRAGMENTATION WITH ECORI ENDONUCLEASE
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DOI：
10.1007/bf00332900
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
BOTTOMLEY, W
中科院分区：
文献类型：
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作者：
ATCHISON, BA;WHITFELD, PR;BOTTOMLEY, W
发展了一种用EcoRI内切酶消化叶绿体DNA的技术，而不需要事先从细胞器中分离DNA。产生的特殊片断图谱表明，不同科[藜科、茄科、葫芦科、豆科、十字花科和裸子植物]或不同属植物的叶绿体DNA几乎没有共同的条带，直到同一属的物种之间进行比较才能发现相似之处。物种间共有的条带数目因属而异，叶绿体DNA的相似程度可能与物种之间的亲缘关系密切相关，根据物种形成可行杂交的能力来判断。
A technique was developed whereby chloroplast DNA was digested with EcoRI [Escherichia coli] endonuclease without prior isolation of the DNA from the organelle. The specific fragmentation patterns produced show that chloroplast DNA from different families [Chenopodiaceae, Solanaceae, Cucurbitaceae, Leguminosae, Cruciferae and Euglenaceae] or genera of plants have few if any bands in common, and it is not until comparisons are made between species of the same genus that similarities become apparent. The number of bands in common between species varies considerably from genus to genus, and the degree of similarity of chloroplast DNA may be correlated with how closely related the species are as judged by their ability to form viable hybrids.