Primase-polymerases: how to make a primer from scratch.
Primase-polymerases: how to make a primer from scratch.
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DOI：
10.1042/bsr20221986
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发表时间：
2023-07-26
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
为了将遗传信息传递给下一代，细胞必须忠实地复制自己的基因组，为每个子细胞提供副本。为了合成这些复制品，细胞使用了被称为DNA聚合酶的特殊酶，这种酶可以快速而准确地复制核酸聚合物。然而，大多数聚合酶缺乏直接启动DNA合成的能力，需要被称为引物的特殊复制酶来制造短的多核苷酸引物，然后它们从那里延伸。复制引物(真核生物和古生菌)属于一个功能不同的酶超家族，称为Primase-Polymerase(Prim-POLS)，其同源基因存在于生命的所有领域。这些酶具有保守的催化Prim-Pol结构域，在DNA新陈代谢中扮演着不同的角色，包括DNA复制、修复和损伤耐受性。这些生物学作用中的许多从根本上是由Prim-Pol产生从头开始的引物的能力所支撑的。这篇综述考察了我们目前对Prim-Pol用来启动底物合成的催化机制的理解。
To pass on genetic information to the next generation, cells must faithfully replicate their genomes to provide copies for each daughter cell. To synthesise these duplicates, cells employ specialised enzymes called DNA polymerases, which rapidly and accurately replicate nucleic acid polymers. However, most polymerases lack the ability to directly initiate DNA synthesis and required specialised replicases called primases to make short polynucleotide primers, from which they then extend. Replicative primases (eukaryotes and archaea) belong to a functionally diverse enzyme superfamily known as Primase-Polymerases (Prim-Pols), with orthologues present throughout all domains of life. Characterised by a conserved catalytic Prim-Pol domain, these enzymes have evolved various roles in DNA metabolism, including DNA replication, repair, and damage tolerance. Many of these biological roles are fundamentally underpinned by the ability of Prim-Pols to generate primers de novo. This review examines our current understanding of the catalytic mechanisms utilised by Prim-Pols to initiate primer synthesis.
影响因子：
8.8
作者：
Liew LP;Lim ZY;Cohen M;Kong Z;Marjavaara L;Chabes A;Bell SD
通讯作者：
Bell SD
古细菌真核型引物酶有效合成引物所必需的引物酶亚基
影响因子：
16.6
作者：
Liu B;Ouyang S;Makarova KS;Xia Q;Zhu Y;Li Z;Guo L;Koonin EV;Liu ZJ;Huang L
通讯作者：
Huang L