Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy
Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy
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DOI：
10.1016/j.biomaterials.2020.120059
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发表时间：
2020-08-01
期刊：
影响因子：
14
通讯作者：
Diao, Jiajie
中科院分区：
文献类型：
--
作者：
Chen, Qixin;Shao, Xintian;Diao, Jiajie
超分辨率光学显微镜将细胞生物学的空间分辨率从细胞水平扩展到纳米尺度，使观察单个线粒体和溶酶体的相互作用行为成为可能。然而，在超分辨率光学显微镜下，线粒体和溶酶体之间相互作用的定量参数化目前是不可用的，这严重限制了我们对线粒体功能的分子机制的理解。在这里，我们引入m值，在结构照明显微镜下定量研究线粒体和溶酶体接触（MLC）和线粒体自噬。我们发现，MLC的m值通常小于0.4，而在有丝分裂中，m值在0.5到1.0之间。该系统允许进一步研究线粒体和溶酶体相互作用的详细分子机制。
Super-resolution optical microscopy has extended the spatial resolution of cell biology from the cellular level to the nanoscale, enabling the observation of the interactive behavior of single mitochondria and lysosomes. Quantitative parametrization of interactions between mitochondria and lysosomes under super-resolution optical microscopy, however, is currently unavailable, which has severely limited our understanding of the molecular machinery underlying mitochondrial functionality. Here, we introduce an M-value to quantitatively investigate mitochondria and lysosome contact (MLC) and mitophagy under structured illumination microscopy. We found that the M-value for an MLC is typically less than 0.4, whereas in mitophagy it ranges from 0.5 to 1.0. This system permits further investigation of the detailed molecular mechanism governing the interactive behavior of mitochondria and lysosomes.